I am humbly asking for some advise or tips to do this kind of methods for experimenting a new staining agent from plants.
And this experimented staining agent is used for the light microscopy only.
I am confused if my study is belong in Plant Physiology research or Horticulture research. My planned study is to determine the growth and nutritional properties of selected leafy plant influence...
05 June 2017 2,431 24 View
Most paper, they use a DSLR Camera for taking a pictures for digital image analysis. I don't have a DSLR Camera because it is expensive to buy. For other alternative, is it reliable to use...
05 June 2017 3,269 0 View
This field makes me interest to do this kind of study.
10 November 2016 4,718 4 View
Is there a signalling molecule which trigger to undergo a photoprotection in C3 Plant? If there is, what kind of gene is this and where it is coded? In cpDNA or nDNA?
07 August 2016 2,216 0 View
If you are looking a Plant Physiology group especially in Facebook, now there it is. The "League of Plant Physiologist" is a non-profit Facebook group and now looking for future and professional...
05 June 2016 6,471 6 View
This time, I am currently search some good topic to be study about Seed Ecology and Physiology. This field gives me a curiosity in order to learn. And, if I already conceptualize your suggested...
01 February 2016 2,566 3 View
Greetings! I am curious about this topic. Most of the research about microgravity is focus on the morphogenesis and tropism of the plant. As far as I know, the microgravity can affect the...
11 December 2015 5,706 5 View
What is the effect of dissolved oxygen on plants in hydroponics system? I am curious about the physiological effect of dissolved oxygen in plants.
10 November 2015 4,719 3 View
I am looking a best statistical software for studying bioassay, ecology and systematics which can help in my research. :)
08 September 2015 1,226 4 View
I am looking for some standard guidelines for identifying the microbiological quality of fresh commodity in the market. I am planning to make a research about this topic. Currently, I am looking...
08 September 2015 6,056 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,336 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,617 3 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,726 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,705 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,911 7 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,733 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,622 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,481 5 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,022 2 View