Is there a tool for 3D miRNA modeling?

For example for an interaction study between a protein target.

22 November 2020 3,952 4 View

New network testing models for deep learning in biological testing ?

We tested four models: logistic regression (LogReg), multilayer perceptron (MLP), graph convolutional network (GCN), and edge-attention graph convolutional network (EAGCN). Are there other models...

08 November 2020 2,347 4 View

Where to find cancer datasets composed by clinical markers and gene expression levels?

Currently, I am validating a new machine learning approach. But, I need to find different cancer datasets composed by clinical markers and gene expressions. For now, a just found MMRF data set...

11 October 2020 9,012 2 View

Can I use a Glass electrode (standard used for pH measurement) for measuring ORP?

I have made a test to investigated: I measured the potential in mV of a standard +220mV solution using my pHmeter which gives a mV value with the pH. The result was +9mV. But this was the result...

06 October 2020 7,220 3 View

How to do validation on a new instrument?

Halloo scientists, please help me the procedure s on doing validation on new bought instrument/equipment before starting doing analysis

07 September 2020 1,590 3 View

Can my RNA interference have any influence in my endougenos control gene?

Good night all. I'm using RNAi to knockdown a transcription factor from a primary cell and I need to validate by westernblot. With Ponceau marking i can see that i'm putting the same amount of...

25 August 2020 263 3 View

How can I hide nodes with posterior probability under 0.5 in BEAST phylogenetic tree visualization?

06 July 2020 1,376 6 View

How do I stored nano particles in my workplace and how long?

I am looking for a technique which is used to store the nano particles without any property change.

23 June 2020 2,473 15 View

List of environmental journals with no publishing fees?

I'm looking for a well-indexed Environmental/ocean science journal to publish my research papers without any publication fee, could you help me?

20 May 2020 2,233 14 View

Validation set error is high in deep learning using Keras/R, how to fix it?

Hello, In these days i'm trying to train a deep neural network with a matrix with 14382 variables x 671 cases, from IV spectral data. I'm using R with Keras package (compile and execution commands...

07 April 2020 8,098 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View