How to validate my flow cytometry protocol for quantifying muscle satellite cells?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

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05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

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05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

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05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

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05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

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04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

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04 August 2024 8,997 1 View

Is there a problem with my RNA pellet?

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11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

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08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

Gromacs first step of minimization ?

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Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 641 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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How to isolate lymphocytes from mouse spleen?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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Derek Li

Maybe a7Inegrin, Vcam-1 double-positive?

Narmadha Murugesan

Okay, I will try these two markers to get accurate number of satellite cells. Thank you for the suggestion Derek Li

Narmadha Murugesan There's a paper that mentioned a7Inegrin, Vcam-1 double-positive cells map over 90% of Pax7-transgened mice satellite cells. I couldn't find it for now. So if the outlier is less than 10%, I think that'll be acceptable.