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Questions related to Blotting Techniques
They've discontinued this polyclonal antibody it and we'd like to repeat some key experiments! We'd be willing to compensate you for it and pay for the shipment. Any help would be greatly appreciated!
07 May 2017 4,587 3 View
05 May 2017 6,788 3 View
I am planning to order an antibody, but I am unsure on which antibody works best as an exosomal marker for DLBCL cell lines. I have read papers suggesting the use of CD63, CD81 and TSG101 commonly...
05 May 2017 6,548 3 View
Hello everyone, I have issues when doing a mycoplasma test with PCR (a routine we set in our lab to test the presence of mycoplasmas in other's cell lines), using a 1% agarose gel. When taking the...
05 May 2017 2,852 3 View
Hello everyone, I'm currently investigating Hippo pathway in ADPKD (Autosomal Polycystic Kidney Disease). We have a very good Ab to detect Yap protein which is also very specific and works nicely...
05 May 2017 4,862 1 View
Hi, I have trouble detecting a protein in a western blot. In my current blots I can’t see any bands at all. In my experiments I am looking at different proteins in unphosphorylated and...
05 May 2017 3,156 11 View
I am trying to probe for a loading control. I don't know why sometimes the bands show up and other times they don't. It's black or white whereas the actual protein I'm tagging for is consistent...
05 May 2017 7,963 3 View
04 May 2017 9,541 3 View
Hello everybody, somebody could suggest or recomend any antibody anti-M13:HRP (pVIII). I think that everybody has been using the one from GE but is discontinued and is not going to be replaced and...
25 April 2017 8,686 20 View
Hi, I am pipetting an antibody with 50% w/v glycerol. However, I need the antibody solution to dry on the nitrocellulose membrane. How can I do this without damaging the antibody? Thank you.
21 April 2017 1,128 1 View
04 April 2017 5,441 21 View
04 April 2017 3,586 1 View
THP-1cells were treated with bacteria , and the three treated group showed two bands on photographic film , while the control group(without bacteria) showed one band . How can I analyze the bands...
04 April 2017 4,489 3 View
Hi guys, Is there any new and efficient western blot apparatus that you recommend?
04 April 2017 6,531 8 View
I stained a nuclear protein (Lamin b1) to control the purity of my samples and the extraction of the nuclear compartment after a nucleo/cytoplasm extraction and it looks nice, but I stained also...
04 April 2017 9,205 6 View
I want to identify detectable change in pH of a drop that has only 25-50 microliters in it. Is there an instrument or method other than pH meter to accomplish this?
31 March 2017 4,297 2 View
I am trying to detect pCreb in skeletal muscle from mice by using WB. But when i did a test i got 2 bands (~45kDa and ~100kDa), instead of one band at 43 kDa. I am using an antibody from Cell...
08 March 2017 3,340 3 View
Hi everybody, Is there any recommended new and fast western blotting apparatus that any of you guys experience with good results and fair price?Thanks a lot
03 March 2017 9,946 6 View
hi cell signalling company recommend that i use 1:1000 dilution for phospho-Akt but at my disposal is only about 1ul of primary antibody left. i usually use a falcon tube at 4ml total primary...
03 March 2017 8,017 0 View
03 March 2017 9,212 2 View
03 March 2017 5,450 3 View
Do you have experience with diffusion blotting from SDS-PAGE? Would you mind sharing a protocol? Prof. Ingrid Olsen shared a great protocol for precast 0.5mm GE Healthcare SDS-PAGE gels in the...
03 March 2017 10,080 2 View
Hi. I have a question regarding MMP-3 detection. Why do most paper use wester blot instead of ELISA? I also found out ELISA kit is also available and the process looks more simple. Thanks in...
20 February 2017 6,623 3 View
02 February 2017 4,229 4 View