What is the best way to measure minor changes of Ph in only 50 microliters of solution?

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Technically: Do proctoring software add to quality assurance in assessment in blended learning modules?

One of the challenges in ensuring quality and integrity in blended learning modules is cheating especially when it comes to assessment (Tests, exams, and assignments). Now one of the solutions...

21 July 2024 6,741 0 View

Did Einstein misunderstand the photo-electric effect?

Photo-electric effect A light particle moving towards a surface cannot (by collision) force an electron particle to move away from that surface. A light wave can instead make interference with a...

19 July 2024 8,389 9 View

Can we find the Gromacs version from XTC file or TRR?

Hello everyone, This soudns like a strange question, but I created five XTC files using five different versions of GROMACS some years ago. All other files are missing, and I want to repeat the...

16 July 2024 7,205 2 View

Two PhD and One Master to expand R&D team, anyone interested?

Hi all, if you are looking for PhD and Master opportunity to work on Microalgae research and Biosensor for bacteria detection. Please do not hesitate to email me [email protected]

07 July 2024 7,576 0 View

Is light quantisation an illusion?

The photo-electric effect does not support quanta in light, since a light particle moving towards a surface cannot cause an electron to move away from the same surface at a right angle. Instead, a...

06 July 2024 2,288 35 View

Is gravity an EMERGENT phenomenon?

Newton found the law of gravity - but did not understand it. Fatio understood the law - but he was not accepted. Fatio assumed fast particles moving in all directions. Stability in planetary...

30 June 2024 9,284 28 View

Any automatic way to outline an image to measure the area in pixels?

Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...

27 June 2024 5,068 1 View

Unstructured Interview Design?

In my qualitative study, I am trying to avoid the traditional belief that types of qualitative research design are just grounded theory, ethnography, phenomenology, case study, historical, and...

15 June 2024 3,884 1 View

Considering the issues with the ERP of GCTU, management has asked that you advise them on cloud computing models.?

Considering the issues with the ERP of GCTU, management has asked that you advise them on cloud computing models. You are expected to give 3 advantages and disadvantages of each of the models....

09 June 2024 5,629 0 View

Can I or you add publications to the page of Adri Jacobs?

Dear ResearchGate, I came across the ResearchGate page of Adri Jacobs, who passed away in 2010. This page contains 25 publication of Adri mainly from the period 2000-2008. One paper (2011) must...

01 June 2024 8,763 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Is there anyway to distinguish two proteins of same molecular weight?

I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...

14 July 2024 9,324 2 View