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Questions related to Biomolecules
I have used following method for the colloidal chitin preparation. Ten grams of chitin was slowly dissolved in 400 ml of concentrated HCl with stirring at 4°C. The mixture was incubated in a water...
04 April 2014 3,991 7 View
I have found bioblender and pymlp.
03 March 2014 1,973 2 View
I would like some learning data sets (WT + a lot of mutants) in relation to half life.
03 March 2014 5,248 0 View
I intercalated a drug with the base pairs AT,AA,GC etc (theoretical work). Then I have calculated the chemical shift values of the base pairs without drug and with drug. There is changes between...
02 February 2014 10,041 2 View
I need a robust assay to determine enzymatic activity of my protein. The two small molecules are not UV-Vis unique. The two small chemical molecules are Naphthelene backbone and an isoprene tail....
02 February 2014 6,214 5 View
Amphotericin B has high affinity to sterol (viz.ergosterol). This interaction can be utilized to quantify amphotericin B (drug). ELISA is one good technique. However, there is very less literature...
01 January 2014 6,498 0 View
Receptors on a cell are more or less accessible for pharmaceuticals depending on their position in the cell membrane and the surrounding environment like carbohydrates etc. Under in-vivo...
01 January 2014 8,369 6 View
I am planning to do in vitro mRNA polyadenylation using Poly(A) polymerase. What are the sequence requirements on the 3´- end of the DNA template downstream of the gene of interest and upstream of...
01 January 2014 4,952 0 View
I am trying to find a data set for analysis which is acquired by CE-MS. In case you have one or you know where I can download one, please do not hesitate to let me know!
01 January 2014 6,055 2 View
Recently, I tried to analyze the transverse magnetization decay curves using distributed exponential fitting analysis according to the regularized inverse Laplace transform, based on the CONTIN...
23 December 2013 8,781 5 View
While simulating protein-ligand interactions, which is the best method for understanding electrostatic interactions?
12 December 2013 6,276 10 View
12 December 2013 8,154 6 View
I would like to draw a DNA structure (both single strand and double strand).
12 December 2013 6,551 4 View
Should I use TEM, SEM or some other microscope?
10 October 2013 8,844 5 View
The links that used to point to the location to download the software are all broken. If these links are no longer correct, does anyone know how I can get this software?
09 September 2013 5,209 1 View
Are there molecules that could be more unstables at -80º C than -20º C. If there is anyone with this behaviour, which is the reason? I can only think in biomolecules like proteins that have...
11 July 2013 2,541 1 View
07 July 2013 2,452 1 View
The compound contains five different kind of natural products .When they are given is as a single compound they are very effective . How to determine the activity of each component in it.
07 July 2013 8,869 0 View
I am trying to dissolve in a free amino acid in the following composition e.g. 270mg L-serine in 14ml acetone:water/50:50 e.g. 73mg L-tyrosine in 14ml chloroform However, none of them can...
16 May 2013 8,699 3 View
Lipids' plasmatic membrane
05 May 2013 6,193 3 View
How can Congo red dye stain cellulose fiber? What are the interactions between the dye and fiber?
05 May 2013 7,872 4 View
I need more than 3 hours to incubate in 56 degree because the sample is not viscous yet.
04 April 2013 5,836 0 View
There is theoretical possibility of disulfide bridges confirmation with Raman frequencies from 485cm-1 to 500cm-1, but it is a bit unusual. There are even experimental proofs for some model...
03 March 2013 3,944 4 View
I'm currently in practical training. My supervisor gave me a project to conduct. Can u help me understand this project? Overexpression of xylanase gene of bacillus (mutant and wild type) into e...
12 December 2012 4,323 1 View