How can Congo red dye stain cellulose fiber? What are the interactions between the dye and fiber?
Hi! Maybe interactions of the amine groups of the dye and of the most accesible OH group of the cellulose?
Mainly due to stacking of dye aromatics with the hydrophobic sugar planes of cellulose. Also, there should be hydrogen bonds as pointed out by Jon Trifol. Check the article cited below
"Cellulose, 2012, 19:1495-1506"
I think the hydrophobic interaction may be the main force and pH doesn't affect the binding of the dye.
Yes, I found HCL and NaoH didn't release any dye from binding.
Unculturable bacteria represent the majority of the microbial world. There are also many bacterial pathogens are very hard to grow in vitro. What are some possible ways to grow those...
08 September 2016 554 3 View
During DNA transformation experiments, we sometimes see a few false positive transformants ---- bacterial colonies that carry no any insert plasmids or DNA fragments but can still grow on...
07 August 2016 3,852 5 View
Genetic manipulation is easier in some model bacteria such E. coli and B. subtilis. However, we need to study many medical and environment related bacteria isolates. What experiencedo you have to...
04 May 2016 2,320 2 View
There are some reports on reducing CO2 into fuel chemicals but the efficiency is quite low. What do you think is the limiting factor of CO2 reduction? We have to identify the rate-limiting factor...
01 February 2016 1,245 6 View
Many proteins are oxygen-sensitive. If I want a lot of those proteins, is there any solution to express and isolate those proteins without loss of their activity?
08 September 2015 4,921 5 View
Thermo-stable proteins are of great industrial importance. Say, I want to increase the stability of a protein by raising its optimal temp from 50 to 70°C. It is relatively easy to create a...
06 July 2015 10,017 7 View
Thermophilic bacteria grow under high temperature but the problem is that many coenzymes (NADPH, NADH) are not very stable under high temperature. What mechanisms do those bacteria use to deal...
04 May 2015 3,009 4 View
Cell-free systems utilize the key enzymes (may need coenzymes/ATP) involved in biochemical reactions/pathways to synthesize desired products in vitro. With the complexity significantly reduced,...
03 April 2015 403 6 View
I am looking for some good academic conference for bioenergy, biofuels and biotechnology field. It would be best if the conference is in the US.
03 April 2015 4,827 0 View
Many enzymes in biochem pathways are in complex form, such as ATP synthase, some fatty acids synthase, enzyme complexes in electron transport chains. How do those proteins expressed in cells? Are...
02 March 2015 9,947 8 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View