12 December 2012 1 4K Report

I'm currently in practical training. My supervisor gave me a project to conduct. Can u help me understand this project?

Overexpression of xylanase gene of bacillus (mutant and wild type) into e coli:

1. How do I perform this experiment? Can you provide a protocol?

2. How many type of vectors do I need for this experiment?

3. How to check the activity of expressed genes?

4. What is meant by gene expression? What do I really need to do by overexpression? How do I overexpress the gene?

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