I'm currently in practical training. My supervisor gave me a project to conduct. Can u help me understand this project?
Overexpression of xylanase gene of bacillus (mutant and wild type) into e coli:
1. How do I perform this experiment? Can you provide a protocol?
2. How many type of vectors do I need for this experiment?
3. How to check the activity of expressed genes?
4. What is meant by gene expression? What do I really need to do by overexpression? How do I overexpress the gene?