What could be the reason behind this discontinuity in UV-Vis spectra?

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Olakunle J Onaolapo

Hope this is not a problem with the diffraction grating.

Hasan Muhammad Usama

Thanks for the answer. If it is indeed a problem with the diffraction grating how can I find out ? What else could it be ?

I don't quite know the details of the spetrometer and it's settings. It's a PerkinElmer LAMBDA 1050 UV/Vis/NIR Spectrophotometer, and the data was obtained in diffuse reflectance mode. That's about all I can tell at this point. And please see the attached image of the original experimental data.