I bought an inhibitor (Jedi1; CAS 108124-17-0 for who wants to check the structure) and my colleague put the poder upon arrival in the freezer instead of the fridge. It was there just over night (16h) and we then put it in the fridge. Should I expect that the reconstituted molecule doesn't work anymore or at a lower concentration or eventually even in a different way? As a biologist I do not know how these temperatures might habìve an impact on the molecules structure or if tehy eventually make molecules stick together so that when I dissolve some molecules will remain sticked together, hence Ill have unequal distribution and concentration.
Tahnks for any explanation or tip (except that I should just buy a new one or try in an experiment, I thought of that but I do not have the time).
If the compound was obtained as a solution, then make sure to mix it thoroughly when you thaw it, since it may have separated from the ice when it froze. Otherwise, there is nothing to worry about.
As you know some samples are less stable than others, if there are concerns about stability then the lower the storage temperature then the the slower the decomposition rate. So either 5C (fridge) or -18c are the norms for long term storage. If a solid/powder as your sample is: then all should be fine. Just allow the sample in its container to warm to room temperature before opening, as it make absorb moisture from the atmosphere. If a solution particularly in DMSO or water or buffer then precipitation can occur at lower temperatures so care is required to ensure complete solution before use.
Your sample may form aggregates in solution, but my view is storing the sample as a powder at -18C won't affect its propensity to form them. But cooling a solution of the powder could.
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