Hello,

I want to analyze the level of activity of some CDK's in tumors (xenograft) that I have snap frozen in liquid nitrogen.

I found this protocol

A new cancer diagnostic system based on a CDK profiling technology. DOI: 10.1016/j.bbadis.2005.06.001

but it seems that I will need a lot of material, which migght be the limiting factor and it is very time consuming.

Did anybody have personal experience with this protocl and can give tips and tricks or suggest other protocols where I need less material?

Thanks in advanece