Dears,

I used 4% PFA to stain in vitro cultured cells for protein staining (Cristal Violet).

No I would like to stain lipid droplets in the cell with oil-red solution.

I thought I will NEED to add a permeabilization agent, but red in publications, people fix just 20min (or even 30min ) in 4% PFA. for example:

Article The effect of chitooligosaccharides on oleic acid-induced li...

I suppose in methanol-free PFA as the alcohol might ruin the lipids but it is not indicated, then I also thought that the polymerized formaldehyde will not be able to penetrate and fix efficiently inside. But they do not add any detergent.

Can anyone give some suggestions ?

Does PFA fix and permeabilize ?

Thanks