Hi,
I fed mice (6N) with HFD (research diets, D12492) and L-NAME (0.5g/l) to get HFpEF mouse model, but those mice didn't gain too much weight.
I know some people also used HFD to establish obesity or other mouse models. How did you feed mice? How often did you replace new HFD? Does anyone have any suggestions about that?
Thanks.
Maybe you did not handle your experimental animal as supposed
Ensure that the experimental conditions such as housing, handling, and food intake measurement are consistent and properly monitored.
Selection of the diet is critical her So we used Normal chaw diet mixed with higher percentage of Lard or saturated Fatty acids from different sources and feeding may continued for at least 16 or 20 weeks
Instead of answering, I'm asking a question: did your HFD mice gain weight as quickly as those in the references? We are facing the same issue at the moment. We ruled out animal resources, the environment and the standard D12492 HFD chow, but the animals still don't have desirable weight gain.
Yan Chen Actually, I haven’t been working with HFD mice for quite some time. But in my experience, only HFD mice gained more weight than HFD+L-NAME mice. We replaced the HFD twice per week, and we also placed some food directly in the cage, not just on the rack.
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