Hello Dr. Nomura

It is cleaving by chemicals (e.g. TFA and scavengers). Sorry for the incomplete information.

Thanks.

Simon Miguel Lopez Dear Simon, if you want to cleave the peptide from the resin and simultaneously cleave the side-chain protecting groups, you can use 2 hours + 1 hour for each arginine. So if you have 4 arginines, you will need 6 hours of cleavage.

Thank Dr. Vrettos for this insight.

I was working on a 90 min + 30 min for every R hybrid protocol as recommended by CEM Liberty Blue technical bulletin and our in-house method using 90% + TFA cleavage cocktail. Our upper limit is that, if ever it exceeded 4 hours, there is a chance that the peptide might be degraded too, aside from being cleaved by the resin. Nonetheless, I will try to apply your recommendation too so that I would be able to yield enough crude synthetic peptide.

Best.