Good Day
Based from your experience, I would like to ask if it is usual that there are dead and floating transfected HEK293 cells after seeding in PLK-coated coverslip?
In my experiments, I used 0.025 mg/mL of PLK to coat a 12 mm coverslip in a 24-well plate. Then, I washed it 4 times. Then, in my seeding experiments, I made sure that (1) I used a warm DMEM medium, (2) gently shake the seeded cells, and (3) minimize the bubble formation during resuspension.
I thought this would reduce the number of dead floating cells, but I still got dead and floating cells that aggregate in the middle. Nonetheless, I still got healthy cells that were attached in the coverslip.
Hoping for your insightful answers.
Thank you.
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