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Questions related from Sanhita Saha
I have isolated a Gram-positive, aerobic bacterium from soil that exhibits promising probiotic and plant growth-promoting properties. I would like to evaluate its potential for pesticide...
29 July 2025 9,666 2 View
I have a little confusion between different terms of "biotics". Can anyone help me to understand the exact meaning of different terms - probiotic, postbiotic, metabiotic, paraprobiotic, prebiotic,...
29 June 2025 2,879 1 View
I am working with extracellular metabolites produced by an isolated bacterium. These metabolites show multiple potential benefits in food preservation, agricultural productivity, and gut health in...
27 June 2025 3,487 3 View
What is the advantage of using bacterial metabolite for application in food preservation compared to other microbial sources?
23 June 2025 3,633 1 View
What is the key difference between targeted metabolomics, untargeted metabolomics, metabolic profiling, metabolic fingerprinting and metabolic footprinting?
21 June 2025 7,821 2 View
Does metabolic footprinting focus on extracellular metabolites, and metabolic fingerprinting focuses on intracellular metabolites? I have a confusion between these two terms.what is key difference...
20 May 2025 5,657 1 View
I have done the outer membrane permeability or NPN assay for gram-negative bacteria (P. aeruginosa). I would like to know if the NPN assay is applicable for gram-positive bacteria such as...
18 May 2025 3,112 2 View
During the preparation of fish food supplemented with chitosan to investigate its impact on the growth and survival of fish, what pH range of chitosan should be maintained to avoid harming the fish?
20 April 2025 6,396 0 View
After centrifuging the broth culture and filtering the supernatant through a 0.22 µm syringe filter, should the resulting filtrate be termed a cell-free supernatant (CFS) or extracellular organic...
10 April 2025 8,323 2 View
I have synthesized a PVA hydrogel using isopropyl alcohol as the gelation agent. For SEM analysis, the hydrogel sample must be converted into a dry powder. However, since the presence of solvents...
26 February 2025 5,057 1 View
I have extracted metabolites from a bacterial culture, lyophilized them, and subsequently suspended the metabolites in sterile water at a concentration of 5 mg/mL. I aim to evaluate the...
09 January 2025 7,445 1 View
I have to study the chitinase activity of a bacteria. Can anyone give me some research articles on this experiment?
11 September 2024 3,689 0 View
To prevent intestinal disorders, probiotics can be rendered multidrug-resistant to survive in the presence of co-administered antibiotics, and they may also transmit the antibiotic-resistance gene...
02 April 2024 8,101 2 View
I would like to perform an untargeted LC-MS analysis of the cell-free supernatant of bacterial culture to analyse all the possible metabolites present in the cell-free supernatant. Here I am...
05 November 2023 6,168 2 View
I have extracellular as well as intracellular metabolite. I have dried the sample both by lyophilizing and air drying at room temperature. It usually takes one week to get the powdered sample...
03 November 2023 9,463 1 View
Up to what percentage DMSO can be used so that it does not have any toxic effect
12 October 2023 9,882 1 View
I have an intracellular metabolite which contains both hydrophilic and hydrophobic compounds. What is the procedure for separating hydrophilic compounds and hydrophobic compounds?
12 October 2023 7,536 3 View
I have extracted metabolite from probiotic bacteria and lyophilized the sample. I have to check the cytotoxicity of that metabolite. Both hydrophilic and hydrophobic compounds are present in the...
05 October 2023 4,076 2 View
I have extracted metabolite from a bacterial cell. I have to check the antimicrobial activity of metabolite. which solvent can I use to dissolve that metabolite as it contains both polar and...
06 June 2023 3,036 3 View
I need to prepare PBS buffer. I would like to know if the functionality of the buffer gets degraded after autoclaving it?
09 March 2023 8,717 1 View
I have to prepare MRS broth and add 0.4% phenol to that media . How can I sterilize this media containing phenol?
05 September 2022 6,997 1 View
I have attached a picture name as Image-1 from the research paper Li et al. 2021. here in Fig-3 this is written that " The different letters (a-d) indicate significant differences between...
17 April 2022 5,810 3 View
I have a bacterial metabolite. I want to identify both polar and non-polar metabolites present in the sample. Do I need to separate metabolites into polar and non-polar categories run separately...
07 March 2022 7,117 5 View
I want to study the LC-MS analysis of a bacterial metabolite.The bacteria is a carotenoid producing bacteria. I have added methanol to the cell pellet and then undergo sonication for cell...
27 February 2022 3,168 4 View
I want to perform an untargeted lc-ms analysis of bacterial metabolite. Generally, we break down the cell of this bacteria by dissolving the pellet with methanol and then give heat treatment for...
20 February 2022 2,939 3 View
