I need to prepare PBS buffer. I would like to know if the functionality of the buffer gets degraded after autoclaving it?
The phosphate might precipitate out but that can be fixed by agitation after it has cooled. Otherwise PBS is mineral in composition with no thermolabile components.
I have design a 6GHz antenna for wifi.The feed line width is 2mm and there are 2 parasitic elements beside this feed line with 1 mm gap.Is there any problem at the time of febrication?
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I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
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Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
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