09 March 2023 1 9K Report

I need to prepare PBS buffer. I would like to know if the functionality of the buffer gets degraded after autoclaving it?

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Hlw, I am trying to build a model a single pile in 3 layers soil in Flac-3D. Could you please help me about the Flac-3D code?

I am facing difficulties to insert the pile in soil layer and define the pile exactly through Flac-3D code.

22 June 2024 8,334 1 View

How to reduce notches in input AC voltage for Totempole Power factor Correction circuit?

Respected readers, I am working with a totem pole PFC circuit. In an experimental study, I face a problem of high-frequency notches in source voltage (Yellow one). I found an explanation behind...

14 May 2024 6,692 6 View

How Wind response spectrum like Davenport implemented on a structure Numerically?

Davenport wind spectrum can calculated through software, but I couldn't understand how it is implemented on a structure through software application. if anybody could enlighten me, it would be a...

05 May 2024 9,728 3 View

How to detect the polymorphism of a material with monolayer thickness?

We have grown boron nitride with a monolayer thickness. XRD usually is used to understand the polymorphism of any material. However, due to the minimum thickness of the sample, it won't work for...

15 April 2024 5,431 1 View

What is the proper procedure of making uniform hydrophobic chrome coated glass wafer surface?

I have deposited chrome on the glass wafer. Then I applied a dielectric layer of SU-8 over it. After that to make the surface hydrophobic, I applied Teflon by using spin coating. However, I found...

12 April 2024 7,009 3 View

Is it good to have antibiotic resistance property for probiotic?

To prevent intestinal disorders, probiotics can be rendered multidrug-resistant to survive in the presence of co-administered antibiotics, and they may also transmit the antibiotic-resistance gene...

01 April 2024 8,043 2 View

Protocol for short term liquid culturing of AML patient derived Peripheral blood mononuclear cells?

Hi everyone! I want to isolate the PBMCs from AML patients and transduce the cells with my lentivirus to knockdown a protein and use for further experiments. I am unable to find a single widely...

14 March 2024 4,317 0 View

How to plot Raman mapping in Origin from raw data?

How to plot Raman mapping data in origin software?

27 February 2024 9,522 0 View

How wind spectrum formed to find wind buffetingloads on vertical slender structure ?

is wind spectrum consume mean wind and turbulence both ? and how the mean wind will be calculated from measured wind data?

04 January 2024 8,546 2 View

Should a specific gene be targeted for comparison or is whole-genome sequencing advisable for a mosquito species lacking a sequence in the NCBI?

What methodologies are involved in sequencing and aligning the genome of a mosquito species for which the sequence is not available in the NCBI? Additionally, how can one construct a phylogenetic...

19 December 2023 6,721 0 View

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Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

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The Optimal Experimental Approach for Gene Function Analysis?

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What is the best blank for nanodrop if I want to read a recombinant protein concentration?

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What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

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EDTA buffer solution?

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Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

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How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

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Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

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