I have extracted metabolites from a bacterial culture, lyophilized them, and subsequently suspended the metabolites in sterile water at a concentration of 5 mg/mL. I aim to evaluate the application of these metabolites in aquaculture by supplementing fish diets.

Could anyone provide insights or recommendations on the following:

Most of the existing studies I have reviewed involve supplementing metabolites in powder form directly into fish feed. However, I need to incorporate the metabolites in liquid form.

  • What is the best method to incorporate a liquid metabolite solution into fish feed to ensure uniform distribution and retention of bioactivity?
  • What concentrations are typically used for such supplementation in experimental diets (e.g., as a percentage of feed weight)?
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