Sanhita Saha To incorporate liquid bacterial metabolites into fish feed effectively, the best methods include 1. spraying or coating the feed with the liquid metabolite solution, 2.mixing the liquid into the feed before pelletizing, 3.using vacuum or rotary coating techniques.

Spraying involves applying the solution onto dry pellets, often with a carrier like oil or molasses, and then drying the feed to ensure even distribution and retention of bioactivity. In the second method, mixing the metabolites with feed ingredients before pelletizing ensures the solution is absorbed during the process, promoting uniform distribution. The third method is done in industrial sector, where using vacuum or rotary coating ensures consistent application of the liquid to the feed surface. In this process, the liquid metabolite solution is sprayed or applied in a controlled environment to the feed as it moves through a drum or chamber.

You can choose one of the first two methods.

For experimental studies, bacterial metabolites in liquid form should be supplemented in concentrations ranging from 0.1% to 1% of the total diet (may go up to 2%). Always start with lower concentrations, monitor fish health closely, and adjust as needed based on experimental outcomes. You can start with concentration of 0.1%-1% of feed weight. Eg., for 1% concentration add 10 grams of the liquid metabolite solution per 1 kg of feed.