Is this 6GHz wifi antenna create complexity at the time of febrication?

I have design a 6GHz antenna for wifi.The feed line width is 2mm and there are 2 parasitic elements beside this feed line with 1 mm gap.Is there any problem at the time of febrication?

04 February 2021 9,241 3 View

How can you make a PEDOT:PSS film using the doctor blade technique?

I have been trying to make an even PEDOT: PSS (PH1000) film with the doctor blade technique (tape casting) with varying results. I have been using a silicon substrate that's treated with piranha...

01 February 2021 8,506 5 View

How robust are open source soil moisture sensors connected to an arduino board for field monitoring?

Seeking recent experiences in the development and deployment of low-cost soil moisture sensors (ideally under $50/sensor) connected to arduino-based dataloggers. What is the longetivity of the...

19 January 2021 6,256 2 View

How to separate a mixture of organic acids by GC?

I would like to develop a method for analysis of many organic acids in the same time. how to separate the chromatographic peaks of each one?

20 December 2020 8,399 3 View

Should I keep the missing values > 50%?

I have a data set of 771 sample size. But some of the variables (laboratory findings like ALT, AST, creatinie level) have more than 50% missing values. Should I drop those? But I think those...

13 December 2020 4,847 3 View

How to Strip off HSQ( Fox 16) after etching?

We are finding a hard time to strip of residual HSQ ( Fox 16) after using it as a mask in etching? We are coating 600 nm thick HSQ and writing with 3000 microC/cm^2 dose at 1 nA current. We are...

08 December 2020 5,452 2 View

Why the oral cancer cell line from metastatic tongue origin is showing less aggressive property?

I am working with oral cancer cell line SCC090 (ATCC). As per ATCC data sheet it is originated from metastatic tongue. It's primary site is not mentioned. Due to the origination from metastatic...

08 December 2020 5,674 3 View

In case of larger sample size (more than 700 sample) can I do t-test though the data is not normally distributed?

My sample size is more than 750 but sk test is showing non-normal distribution. The variables have several outliers but I shouldn't remove those. In that case what should I do? t-test or other...

06 December 2020 1,285 19 View

Why the western blot of HIF1alpha of the whole cell lysate shows intense band under normoxia without any induction of hypoxia?

I am working with oral cancer cell lines and trying to find the expressional changes of HIF1alpha under different experimental treatment. I am getting intense band of HIF1alpha in the western blot...

29 November 2020 5,707 3 View

How to calculate in-plane lattice strain for 2D material?

I am working with h-BN and need to find out the strain due to in-plane lattice constant. The famous Williamson-Hall method may not be applicable here as it analyzes XRD for calculation of strain...

19 October 2020 7,386 4 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

03 February 2021 9,478 2 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

How to get phenol(BHT) back to organic phase from acidic aqueous layer?

07 January 2021 6,527 3 View

What is the best way to sterilize carboxyl-functionalized carbon nanotubes to be used for cell culture?

I want to incorporate CNT in the alginate solution

23 December 2020 9,521 3 View

In microbiology to do plating is there any need to sterile the DI water also?

In microbiology to do plating, is there any need to sterile the DI water also?

22 December 2020 3,288 1 View

What are some good polymer to use for packaging a biological product ,which can be steril by gamma?

As different polymers with different characteristics are exist,I want to know What polymers can be used for packaging an orthobiologic product(bone allograft),which can have enough resistent to...

22 December 2020 8,196 5 View

Sterile chitosan folat polymer?

Hi, I want to start my cell culture to run a MTT assay , how can I sterilize my polymer?

15 December 2020 1,813 1 View