How to do sterilization of MRS broth containing 0.4% phenol?

05 September 2022 1 7K Report

I have to prepare MRS broth and add 0.4% phenol to that media . How can I sterilize this media containing phenol?

Did you try UV sterilisation or radiation sterilization (i.e. gamma rays or electron beams). Radiation could be aggressive in aqueous medium, but performing irradiation on frozen state could help.

SIncerely,

Nicolas

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