Samah Mohammed Refay

8 Questions 16 Answers 0 Followers

Questions related from Samah Mohammed Refay

What is the best method to prepare Sudan black B to dye the oil in yeast?

Hello everyone, I want to determine the yeast producers of oil, I want to know the best method to prepare Sudan black use it. I prepared it in 70% ethanol and hot it, and filter it but when I use...

28 April 2022 1,000 0 View

Using of sucrose instead of dextrose in YPD medium?

Hello everyone, What is the weight of sucrose can I use instead of dextrose in the YPD medium if dextrose is unavailable?

19 March 2022 2,975 3 View

How to prepare a lyophilized cell line leucocyte for DNA extraction?

I have a lyophilized cell line leucocyte, I want to extract DNA from it. How do I prepare it? Dissolve it in water then harvest cells for the extraction or directly dissolve it in PBS.

07 November 2021 2,479 3 View

Could You Suggest some current hot topics in medical Microbiology (bacteriology) for PhD?

I want to start my Phd, I am interested in medical bacteriology and molecular biology. I am interested in infectious diseases, antibiotic resistance, and vaccine development can you advise me on...

27 October 2021 5,121 4 View

How to determine the types of gene resistance of beta-lactamase to perform to my samples by PCR?

Greet to everyone. I did antibiotic resistance by vitek2 compact and I found beta-lactamase resistance phenotypically in Gram-negative bacteria. How can I determine types of bet-lactamase genes to...

06 September 2020 9,014 5 View

How to obtain unstandarizied coefficient in linear regression?

I have a continuous dependent variable and five independent variables(categorical), I want to perform linear regression, I want to obtain the unstandardized coefficient for parameters of the...

02 May 2020 8,329 6 View

Are there any guidelines to choose antibiotic genes resistance for beta-lactam to do multiplex PCR?

I isolated some Gram-negative isolates that appear high resistance to most antibiotics. I want to do multiplex PCR to isolates for beta-lactam gene resistance. are there any recommendations to...

23 February 2020 4,994 6 View

Does boiling method for extraction of bacterial DNA is suitable to obtain bacterial and plassmid DNA to work on antibiotic resistance genes?

I work on antibiotic resistance genes in bacteria. Resistance genes present on plasmid. I extract DNA using boiling method. Does this method suitable to obtain plasmid DNA to study resistance...

05 April 2019 6,748 15 View