18 Questions 29 Answers 0 Followers
Questions related from Samah Mohammed Refay
Hello I have some XDR and PDR bacteria, when i test ESBL production by combined disc method, the bacteria was resistance to cefotaxime and also resistant to combined disc cefotaxime/clavulanic...
18 March 2025 9,470 3 View
I want to calculate lethal time 50, how to determine it by probit analysis in SPSS?
23 February 2025 7,425 2 View
Hello Everyone, I work on VITEK2compact for isolates identification and sensitivity test, I have some problems with device. 1- Some results of sensitivity tests not appear any phenotypic result,...
25 January 2025 980 0 View
I isolated pathogenic bacteria from cancer patients, I will isolate plasmid DNA from these bacteria. I want to design suitable primers to detect ESBLs on plasmid, How and What is the suitable tool...
05 December 2024 1,491 1 View
Dear community I want to detect some genes of ESBLs in bacteria, what the best to detect it in genomic DNA or plasmid DNA?
06 October 2024 1,053 3 View
Hello, If I have the ROC curve from SPSS output, How can I determine the cut off?
02 June 2024 9,720 3 View
Hello every one, I run binary logistic regression in SPSS but i did not have results because of complete separation. How can i solve this proplem? Thanks in advance.
28 May 2024 9,544 11 View
Hello every one Can any one suggest me trends subjects in viral infection in cancer patients for PHD thesis? Thanks in advance.
06 May 2024 5,306 3 View
Hello everyone, I want to determine the yeast producers of oil, I want to know the best method to prepare Sudan black use it. I prepared it in 70% ethanol and hot it, and filter it but when I use...
28 April 2022 1,049 0 View
Hello everyone, What is the weight of sucrose can I use instead of dextrose in the YPD medium if dextrose is unavailable?
19 March 2022 3,030 3 View
I have a lyophilized cell line leucocyte, I want to extract DNA from it. How do I prepare it? Dissolve it in water then harvest cells for the extraction or directly dissolve it in PBS.
07 November 2021 2,536 3 View
I want to start my Phd, I am interested in medical bacteriology and molecular biology. I am interested in infectious diseases, antibiotic resistance, and vaccine development can you advise me on...
27 October 2021 5,173 4 View
Greet to everyone. I did antibiotic resistance by vitek2 compact and I found beta-lactamase resistance phenotypically in Gram-negative bacteria. How can I determine types of bet-lactamase genes to...
06 September 2020 9,119 5 View
I have a continuous dependent variable and five independent variables(categorical), I want to perform linear regression, I want to obtain the unstandardized coefficient for parameters of the...
02 May 2020 8,397 6 View
I isolated some Gram-negative isolates that appear high resistance to most antibiotics. I want to do multiplex PCR to isolates for beta-lactam gene resistance. are there any recommendations to...
23 February 2020 5,104 6 View
Can you suggest me a method for extraction of genomic bacterial DNA from whole blood? How long can i preserve my samples of whole blood at -80 before extraction of bacterial DNA without any...
09 April 2019 1,213 1 View
I work on antibiotic resistance genes in bacteria. Resistance genes present on plasmid. I extract DNA using boiling method. Does this method suitable to obtain plasmid DNA to study resistance...
05 April 2019 6,876 15 View
I work on gene resistance of antibiotics in bacteria and confirmation by pcr How can i obtain positive control strain for pcr work and from For example, I need a positive control strain of...
09 December 2018 3,429 7 View
