Samah Mohammed Refay

13 Questions 26 Answers 0 Followers

Questions related from Samah Mohammed Refay

How can I determine the cut off from the ROC curve?

Hello, If I have the ROC curve from SPSS output, How can I determine the cut off?

02 June 2024 9,619 3 View

What is solution for complete separation in binary logistic regression?

Hello every one, I run binary logistic regression in SPSS but i did not have results because of complete separation. How can i solve this proplem? Thanks in advance.

28 May 2024 9,450 11 View

What are Trends in viral infection among cancer patients?

Hello every one Can any one suggest me trends subjects in viral infection in cancer patients for PHD thesis? Thanks in advance.

06 May 2024 5,131 3 View

What is the best method to prepare Sudan black B to dye the oil in yeast?

Hello everyone, I want to determine the yeast producers of oil, I want to know the best method to prepare Sudan black use it. I prepared it in 70% ethanol and hot it, and filter it but when I use...

28 April 2022 1,009 0 View

Using of sucrose instead of dextrose in YPD medium?

Hello everyone, What is the weight of sucrose can I use instead of dextrose in the YPD medium if dextrose is unavailable?

19 March 2022 2,982 3 View

How to prepare a lyophilized cell line leucocyte for DNA extraction?

I have a lyophilized cell line leucocyte, I want to extract DNA from it. How do I prepare it? Dissolve it in water then harvest cells for the extraction or directly dissolve it in PBS.

07 November 2021 2,486 3 View

Could You Suggest some current hot topics in medical Microbiology (bacteriology) for PhD?

I want to start my Phd, I am interested in medical bacteriology and molecular biology. I am interested in infectious diseases, antibiotic resistance, and vaccine development can you advise me on...

27 October 2021 5,132 4 View

How to determine the types of gene resistance of beta-lactamase to perform to my samples by PCR?

Greet to everyone. I did antibiotic resistance by vitek2 compact and I found beta-lactamase resistance phenotypically in Gram-negative bacteria. How can I determine types of bet-lactamase genes to...

06 September 2020 9,038 5 View

How to obtain unstandarizied coefficient in linear regression?

I have a continuous dependent variable and five independent variables(categorical), I want to perform linear regression, I want to obtain the unstandardized coefficient for parameters of the...

02 May 2020 8,335 6 View

Are there any guidelines to choose antibiotic genes resistance for beta-lactam to do multiplex PCR?

I isolated some Gram-negative isolates that appear high resistance to most antibiotics. I want to do multiplex PCR to isolates for beta-lactam gene resistance. are there any recommendations to...

23 February 2020 5,017 6 View

What is the methods for extraction of bacterial DNA from whole blood?

Can you suggest me a method for extraction of genomic bacterial DNA from whole blood? How long can i preserve my samples of whole blood at -80 before extraction of bacterial DNA without any...

09 April 2019 1,166 1 View

Does boiling method for extraction of bacterial DNA is suitable to obtain bacterial and plassmid DNA to work on antibiotic resistance genes?

I work on antibiotic resistance genes in bacteria. Resistance genes present on plasmid. I extract DNA using boiling method. Does this method suitable to obtain plasmid DNA to study resistance...

05 April 2019 6,770 15 View

I need to know from i obtain a positive control of klebsiella pneumoniae for shv gene?

I work on gene resistance of antibiotics in bacteria and confirmation by pcr How can i obtain positive control strain for pcr work and from For example, I need a positive control strain of...

09 December 2018 3,363 7 View