How we can correct the slide?

i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...

14 February 2021 6,697 1 View

Calculating the CFU?

i am constructing research for the antibacterial activity of the medication and I am testing it by %RCC, so my Q after I did 10 serial dilutions for the bacterial suspension can we do plating for...

04 February 2021 5,886 1 View

How to determine the types of gene resistance of beta-lactamase to perform to my samples by PCR?

Greet to everyone. I did antibiotic resistance by vitek2 compact and I found beta-lactamase resistance phenotypically in Gram-negative bacteria. How can I determine types of bet-lactamase genes to...

05 September 2020 8,898 5 View

Hi, I want to run simulation with YASARA for different part of my protein separately but I dont know how to run?

I am working on enzyme engineering and want to run the simulation for my enzyme the open and closed conformation to see the effect of the mutation on my enzyme as its located on the lid. However,...

10 May 2020 2,625 1 View

Are there any guidelines to choose antibiotic genes resistance for beta-lactam to do multiplex PCR?

I isolated some Gram-negative isolates that appear high resistance to most antibiotics. I want to do multiplex PCR to isolates for beta-lactam gene resistance. are there any recommendations to...

22 February 2020 4,842 6 View

Is there a way to transform a function of two variables into one variable?

Hello, Assume that I have Z=f(x)+g(y). Is there a way that I can write Z as a function of one variable w that relates f(w) and g(w)? Thanks in advance for your help,

29 August 2019 3,132 1 View

Does boiling method for extraction of bacterial DNA is suitable to obtain bacterial and plassmid DNA to work on antibiotic resistance genes?

I work on antibiotic resistance genes in bacteria. Resistance genes present on plasmid. I extract DNA using boiling method. Does this method suitable to obtain plasmid DNA to study resistance...

04 April 2019 6,639 15 View

Sample size in experimental probability?

according to the probability theory, suppose that we calculate the experimental probability of students who prefer mathematics and it was .70%from a sample of 20 students (14/20), is that correct...

06 January 2019 3,681 6 View

Is international journal of innovative research in information security a recognized journal? or it is a fake one?

I want to publish a paper at a journal but I am confused where my experience is not too good to differentiate between fake journals or not.

22 December 2018 4,472 2 View

What is the best taxon sampling that lead to draw a reliable conclusion about the floristic affinities of entire flora?

I'm interested in studying the phylogenetic affinities of the flora of arid, nearshore archipelago (less than 100 km from nearest mainland). 300 species fall in 129 genera were recorded, no...

18 September 2016 499 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View