I work on gene resistance of antibiotics in bacteria and confirmation by pcr
How can i obtain positive control strain for pcr work and from
For example, I need a positive control strain of klebsiella pneumonia for SHV gene
In your samples if you have a positive case for your gene, you have to send it for sequencing to prove that is exactly your gene as a result you can use it for positive control for good.
You should get these control strains from collection centers like ATCC, Microbial culture collection
Sunagar Raju Amal Awad
How can i communicate with ATCC or microbial culture collection?
Samah Mohammed Refay
Go to their website and looking for your gene in Klebsiella strains also there is contact with us part
Your sample probably have a positive strain, once this gene is intrinsic in most of isolates.
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