How we can correct the slide?

i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...

14 February 2021 6,697 1 View

Calculating the CFU?

i am constructing research for the antibacterial activity of the medication and I am testing it by %RCC, so my Q after I did 10 serial dilutions for the bacterial suspension can we do plating for...

04 February 2021 5,886 1 View

How to determine the types of gene resistance of beta-lactamase to perform to my samples by PCR?

Greet to everyone. I did antibiotic resistance by vitek2 compact and I found beta-lactamase resistance phenotypically in Gram-negative bacteria. How can I determine types of bet-lactamase genes to...

05 September 2020 8,898 5 View

Hi, I want to run simulation with YASARA for different part of my protein separately but I dont know how to run?

I am working on enzyme engineering and want to run the simulation for my enzyme the open and closed conformation to see the effect of the mutation on my enzyme as its located on the lid. However,...

10 May 2020 2,625 1 View

Are there any guidelines to choose antibiotic genes resistance for beta-lactam to do multiplex PCR?

I isolated some Gram-negative isolates that appear high resistance to most antibiotics. I want to do multiplex PCR to isolates for beta-lactam gene resistance. are there any recommendations to...

22 February 2020 4,842 6 View

Is there a way to transform a function of two variables into one variable?

Hello, Assume that I have Z=f(x)+g(y). Is there a way that I can write Z as a function of one variable w that relates f(w) and g(w)? Thanks in advance for your help,

29 August 2019 3,132 1 View

Does boiling method for extraction of bacterial DNA is suitable to obtain bacterial and plassmid DNA to work on antibiotic resistance genes?

I work on antibiotic resistance genes in bacteria. Resistance genes present on plasmid. I extract DNA using boiling method. Does this method suitable to obtain plasmid DNA to study resistance...

04 April 2019 6,639 15 View

Sample size in experimental probability?

according to the probability theory, suppose that we calculate the experimental probability of students who prefer mathematics and it was .70%from a sample of 20 students (14/20), is that correct...

06 January 2019 3,681 6 View

Is international journal of innovative research in information security a recognized journal? or it is a fake one?

I want to publish a paper at a journal but I am confused where my experience is not too good to differentiate between fake journals or not.

22 December 2018 4,472 2 View

What is the best taxon sampling that lead to draw a reliable conclusion about the floristic affinities of entire flora?

I'm interested in studying the phylogenetic affinities of the flora of arid, nearshore archipelago (less than 100 km from nearest mainland). 300 species fall in 129 genera were recorded, no...

18 September 2016 499 3 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View

How to design an Ultrafiltration membrane for a CSTR?

Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...

27 January 2021 7,924 1 View

Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

How to overcome problems with introduction of modified DNA into C. glutamicum?

09 December 2020 7,715 5 View

Thin cut brain tissue flaking off of slides?

Hello! I have just encountered a new issue with some tissue flaking off of my slides and wondered if anyone else has experienced this or has any suggestions? The sections were cut at 18 microns...

09 December 2020 4,954 2 View

How to sterilise maize seeds in the lab?

What is the most effective protocol to sterilise maize seeds in the lab? We have tried few common procedures but with no luck (still get fungus contamination on agar plates): 1)10% sodium...

19 November 2020 602 15 View

How to calculate the % yield/conversion of Sucrose fatty acid ester by using GC, GCMS, HPLC and other?

Hi All, How to calculate the % yield/conversion of Sucrose fatty acid ester by using GC, GCMS, HPLC, and others? Please suggest.

19 November 2020 2,645 2 View