Hello everyone,
I want to determine the yeast producers of oil, I want to know the best method to prepare Sudan black use it.
I prepared it in 70% ethanol and hot it, and filter it but when I use it, it was not good and precipitated on the slide.
i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...
14 February 2021 6,697 1 View
i am constructing research for the antibacterial activity of the medication and I am testing it by %RCC, so my Q after I did 10 serial dilutions for the bacterial suspension can we do plating for...
04 February 2021 5,886 1 View
Greet to everyone. I did antibiotic resistance by vitek2 compact and I found beta-lactamase resistance phenotypically in Gram-negative bacteria. How can I determine types of bet-lactamase genes to...
05 September 2020 8,898 5 View
I am working on enzyme engineering and want to run the simulation for my enzyme the open and closed conformation to see the effect of the mutation on my enzyme as its located on the lid. However,...
10 May 2020 2,625 1 View
I isolated some Gram-negative isolates that appear high resistance to most antibiotics. I want to do multiplex PCR to isolates for beta-lactam gene resistance. are there any recommendations to...
22 February 2020 4,842 6 View
Hello, Assume that I have Z=f(x)+g(y). Is there a way that I can write Z as a function of one variable w that relates f(w) and g(w)? Thanks in advance for your help,
29 August 2019 3,132 1 View
I work on antibiotic resistance genes in bacteria. Resistance genes present on plasmid. I extract DNA using boiling method. Does this method suitable to obtain plasmid DNA to study resistance...
04 April 2019 6,639 15 View
according to the probability theory, suppose that we calculate the experimental probability of students who prefer mathematics and it was .70%from a sample of 20 students (14/20), is that correct...
06 January 2019 3,681 6 View
I want to publish a paper at a journal but I am confused where my experience is not too good to differentiate between fake journals or not.
22 December 2018 4,472 2 View
I'm interested in studying the phylogenetic affinities of the flora of arid, nearshore archipelago (less than 100 km from nearest mainland). 300 species fall in 129 genera were recorded, no...
18 September 2016 499 3 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...
28 February 2021 2,955 2 View
I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...
27 February 2021 5,383 3 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?
24 February 2021 8,219 2 View
Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?
24 February 2021 7,545 2 View
I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...
23 February 2021 7,868 2 View
How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex
22 February 2021 5,667 2 View