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Questions related from Sadia Kanwal
Im looking for Graphpad prism full version. can anyone help me with free download.I searched online but i got the trail version.Please help me to get the full version. Many thanks Best Regards...
04 April 2018 4,629 12 View
What is the main difference between Shannon-Wiener Index and Simpson Index.The definition for both the indices is pretty similar. secondly which index is more appropriate for gut microbial...
03 March 2018 1,961 2 View
I got my sequencing results of DGGE(bands) .I'm using SNAPGENE software to retrieve sequences and BLAST in NCBI.I'm not sure if its the correct way to identify bacterial strains.secondly what's...
02 February 2018 8,840 5 View
Which blocking agent gives more clearer result for Internal control antibody like beta actin ?
01 January 2018 8,616 0 View
If we want to assess gut permeability, which is the ideal assay to determine Endotoxemia? Does site of blood collection influence the assay?please suggest . Thanks in advance
01 January 2018 2,354 0 View
How I can eliminate smiley(curve bands) on the edge wells in dgge (usually first and last well). Is there any way that I can get straight and clear bands.
11 November 2017 9,665 1 View
I want to show structure of different polysaccharides in my manuscript, structures i have obtained by searching on google. My question is how i can cite the structure searched in this way? or i...
11 November 2017 9,703 6 View
Does Sanger sequencing gives clear reads for PCR amplified product followed by purification.can we get precise information about the nucleotide sequencing? Suggestions Please :) Best Regards
11 November 2017 7,676 8 View
Does FITC-dextran assay has Toxic effect in mice if administered orally before decapitation? does it will effect other tissues and organs? or is there any alternative method(in vitro assay) to...
11 November 2017 8,446 7 View
What are the critical physico-chemical characterization that should be considered for a novel polysaccharide isolated from mushroom? Thanks
11 November 2017 9,083 3 View
Brightness or contrast of an image can be adjusted by using PS for scientific publications? Is it legal to adjust using this software for publication ?
10 October 2017 1,986 7 View
How to find the total polysaccharide concentration from mushroom powder prior polysaccharide purification? Thanks
10 October 2017 1,058 4 View
How informative is the use of mouse model for human gut microbiota research? What are the pitfalls and how far we are in mimicking the mouse with human gut? thanks
10 October 2017 1,237 3 View
I'm using CLIQS software to analyse DGGE bands.Can any one tell me how to generate a dendrogram using this software ? Thanks
09 September 2017 9,760 0 View
Two bands of Bcl-2 are appearing on my western blot, what could be the possible reason for that? is it normal?
07 July 2017 7,151 7 View
How purity (%) of protein can be determined by densitometry of coomassie brilliant blue stained SDS-PAGE gel ?
07 July 2017 8,682 5 View
For the stepwise and overall yield of the protein, this table makes sense? i am confuse about the yield after refolding and concentration step, because refolding we are dring by direct dilution...
07 July 2017 4,619 3 View
how to validate the transfer condition (time/V or mA) for proteins of different molecular weight in western blotting?what could be the best transfer condition for proteins 14-30 KDa and 30-100KDa?
06 June 2017 9,739 2 View
Hello everyone If the protein concentrations of my samples are 1.039ug/ul, .638ug/ul and 0.511ug/ml.....and i want to load 40ug/20ul/well, how can i proceed ? Thanks
06 June 2017 4,061 6 View
Hello I am working on a recomnibant protein, after its solublization i refolded the protein by using buffers of different composition and pH. In order to determine which buffer is resulting in the...
06 June 2017 4,317 3 View
After DSF guided refolding of protein, results for denatured and refolded samples are coming out like this. is it ok if the denatured sample give peak and Tm like this? as i have used mild...
06 June 2017 8,734 4 View
Any research paper on DSF guided refolding of novel protein ? other then this one https://www.nature.com/articles/srep18906.pdf Thanks Regards
06 June 2017 9,768 0 View
Which protocol i can use to isolate protein from mitochondria (COX IV and cyto c?
06 June 2017 2,635 3 View
I am working on a recombinant protein that belongs to YjgF/Yer057p/UK114 family. I want to know the similarity of my protein with other membrs of this family. Which database i can use, and whar...
05 May 2017 9,030 7 View
I am trying to perform DNA fragmentation assay after drug induced apoptosis in HL-60 cell line, but unable to get good DNA ladder on agarose gel. Any inhouse method for isolation of DNA fragments...
05 May 2017 2,901 6 View
Is there any web server or database through which one can predict the interaction/binding of our protein with RNA using protein sequence?
05 May 2017 1,401 4 View
I want to analyse gut microbial community from faecal samples. since its difficult to sequence all the bands in gel. so I gotta know if there is any free software I can use. Thanks .
01 January 1970 4,822 6 View
