I am trying to perform DNA fragmentation assay after drug induced apoptosis in HL-60 cell line, but unable to get good DNA ladder on agarose gel. Any inhouse method for isolation of DNA fragments from Hl-60 cells?
Dear Sadia Kanwal
Pls. find the attached files and the references, good luck
Hoping this will be helpful to your project
Regards
Prof. Houda Kawas
https://books.google.com/books?id=XdHUBwAAQBAJ&pg=PA121&lpg=PA121&dq=isolation+of+DNA+fragments+from+Hl-60+cells&source=bl&ots=WrTuhquaYP&sig=tbEhESH3XXhMlTZSaRO3wiFmrG8&hl=ar&sa=X&ved=0ahUKEwiE0LLww4_UAhWJFywKHXpZAokQ6AEIbTAI#v=onepage&q=isolation%20of%20DNA%20fragments%20from%20Hl-60%20cells&f=false
https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/carcin/20/5/10.1093_carcin_20.5.851/1/200851.pdf?Expires=1495963089&Signature=MsvS66beTuc8M1FmgVn3bzK9VX5q2-a3r~31tD79usqL9DCAXSClUo149nh~aHvkAmYkAXUmJzDslFcEc6uIEHZSKaKY5WcxgQz8rbp8Q47CU9Kft8LQL55FHmFhklAioS2G~E32xHcpFoc0SPgAEDhbqfGpCa2nFSFRLUgsGOhBsFBZ4sYmIM5I7Fr-7SWfd861fgtfsuYxUfa4VztoF7fMcvt2XfsHhTKzZ3XI9VQZ7GQREe9iTrV5B10bRrtRsTMNz~CMJGATCNeeCJ6ACTU4PsCvE0AEUtHsE~DnIljJpOLcSeONMjXx4DprsBDRqTHpVrMlbo0TtkqO81d6ig__&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q
http://www.jimmunol.org/content/159/11/5412
http://pubs.acs.org/doi/pdf/10.1021/bi00050a033
Article A Selective Procedure for DNA Extraction from Apoptotic Cell...
thank you so much Prof. Houda Kawas
Also
..
Hoping this will be helpful to your project, good luck
https://www.thermofisher.com/iq/en/home/life-science/cell-analysis/cell-viability-and-regulation/apoptosis/dna-fragmentation.html
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