Graphpad prism software full version?

Im looking for Graphpad prism full version. can anyone help me with free download.I searched online but i got the trail version.Please help me to get the full version. Many thanks Best Regards...

03 April 2018 4,492 12 View

Is there any difference between shannon weiner index and simpson index?

What is the main difference between Shannon-Wiener Index and Simpson Index.The definition for both the indices is pretty similar. secondly which index is more appropriate for gut microbial...

02 March 2018 1,778 2 View

Dgge sequence analysis using SNAPGENE?

I got my sequencing results of DGGE(bands) .I'm using SNAPGENE software to retrieve sequences and BLAST in NCBI.I'm not sure if its the correct way to identify bacterial strains.secondly what's...

01 February 2018 8,731 5 View

LPS assay to determine endotoxemia in mice model?

If we want to assess gut permeability, which is the ideal assay to determine Endotoxemia? Does site of blood collection influence the assay?please suggest . Thanks in advance

31 December 2017 2,264 0 View

Skimmed milk or BSA blocking?

Which blocking agent gives more clearer result for Internal control antibody like beta actin ?

31 December 2017 8,520 0 View

Is it necessary to isolate and amplify clones before Sanger sequencing?

Does Sanger sequencing gives clear reads for PCR amplified product followed by purification.can we get precise information about the nucleotide sequencing? Suggestions Please :) Best Regards

10 November 2017 7,534 8 View

Analysis of Intestinal Permeability in mice through FITC-dextran method?

Does FITC-dextran assay has Toxic effect in mice if administered orally before decapitation? does it will effect other tissues and organs? or is there any alternative method(in vitro assay) to...

10 November 2017 8,319 7 View

Physico chemical characterization of Polysaccharide?

What are the critical physico-chemical characterization that should be considered for a novel polysaccharide isolated from mushroom? Thanks

10 November 2017 8,919 3 View

Structure of Polysaccharide?

I want to show structure of different polysaccharides in my manuscript, structures i have obtained by searching on google. My question is how i can cite the structure searched in this way? or i...

10 November 2017 9,598 6 View

How to get the best DGGE gel image?

How I can eliminate smiley(curve bands) on the edge wells in dgge (usually first and last well). Is there any way that I can get straight and clear bands.

10 November 2017 9,570 1 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View