Markers for GC B cells

More Patrick Kao's questions See All

Collagen coating

Does ECM affects cell growth or other properties? Could lacking of ECM or disruption of its structure alter the cell biology?

08 September 2012 7,942 2 View

ELISA of tongue

Has anyone ever extracted protein from tongue tissue to perform ELISA? What kind of lysis buffer did you use?

07 August 2012 6,445 4 View

Quantification of Ki67

Does anyone know how to quantify the result of Ki-67 labelling?

06 July 2012 3,366 23 View

Mouse primer

Can anyone provide me good primers of TNFa, IL-10, IL-5, IL-13, RANTES, IL-17A for Sybr green qPCR use?

05 June 2012 5,617 4 View

Mast Cell Depletion

Does anyone know how to deplete mast cell in vivo? Is there any known antibody can serve this purpose?

05 June 2012 2,791 16 View

Fibrosis detection

How can I detect fibrosis within a tissue? Any staining to apply on biopsy or other methods?

05 June 2012 1,781 15 View

Regenerating marker

To assess the recovery of tongue epithelial cell, what are the marker to look into? Ki67?

04 May 2012 9,093 4 View

Cd34

In which cells is CD34 expressed? What's its function?

04 May 2012 1,000 13 View

COX 2 (cyclooxygenase 2) primer

Have anyone used the primer for qPCR to detect cyclooxygenase 2? May I learn the sequence of them??

01 February 2012 2,715 4 View

NCBI : This record was replaced with Gene ID: 17386

I was looking for the primer of "MMP1" of mouse, yet it shows the message as the title and redirect me to the page named "MMP13", does it means they are the same in mouse?

01 February 2012 7,028 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View