Details of mouse strain

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Collagen coating

Does ECM affects cell growth or other properties? Could lacking of ECM or disruption of its structure alter the cell biology?

08 September 2012 7,942 2 View

ELISA of tongue

Has anyone ever extracted protein from tongue tissue to perform ELISA? What kind of lysis buffer did you use?

07 August 2012 6,445 4 View

Quantification of Ki67

Does anyone know how to quantify the result of Ki-67 labelling?

06 July 2012 3,366 23 View

Mouse primer

Can anyone provide me good primers of TNFa, IL-10, IL-5, IL-13, RANTES, IL-17A for Sybr green qPCR use?

05 June 2012 5,617 4 View

Mast Cell Depletion

Does anyone know how to deplete mast cell in vivo? Is there any known antibody can serve this purpose?

05 June 2012 2,791 16 View

Fibrosis detection

How can I detect fibrosis within a tissue? Any staining to apply on biopsy or other methods?

05 June 2012 1,781 15 View

Regenerating marker

To assess the recovery of tongue epithelial cell, what are the marker to look into? Ki67?

04 May 2012 9,093 4 View

Cd34

In which cells is CD34 expressed? What's its function?

04 May 2012 1,000 13 View

COX 2 (cyclooxygenase 2) primer

Have anyone used the primer for qPCR to detect cyclooxygenase 2? May I learn the sequence of them??

01 February 2012 2,715 4 View

NCBI : This record was replaced with Gene ID: 17386

I was looking for the primer of "MMP1" of mouse, yet it shows the message as the title and redirect me to the page named "MMP13", does it means they are the same in mouse?

01 February 2012 7,028 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View