Details of mouse strain

More Patrick Kao's questions See All

Collagen coating

Does ECM affects cell growth or other properties? Could lacking of ECM or disruption of its structure alter the cell biology?

08 September 2012 8,033 2 View

ELISA of tongue

Has anyone ever extracted protein from tongue tissue to perform ELISA? What kind of lysis buffer did you use?

07 August 2012 6,521 4 View

Quantification of Ki67

Does anyone know how to quantify the result of Ki-67 labelling?

06 July 2012 3,449 23 View

Mouse primer

Can anyone provide me good primers of TNFa, IL-10, IL-5, IL-13, RANTES, IL-17A for Sybr green qPCR use?

05 June 2012 5,704 4 View

Fibrosis detection

How can I detect fibrosis within a tissue? Any staining to apply on biopsy or other methods?

05 June 2012 1,869 15 View

Mast Cell Depletion

Does anyone know how to deplete mast cell in vivo? Is there any known antibody can serve this purpose?

05 June 2012 2,878 16 View

Regenerating marker

To assess the recovery of tongue epithelial cell, what are the marker to look into? Ki67?

04 May 2012 9,165 4 View

Cd34

In which cells is CD34 expressed? What's its function?

04 May 2012 1,103 13 View

COX 2 (cyclooxygenase 2) primer

Have anyone used the primer for qPCR to detect cyclooxygenase 2? May I learn the sequence of them??

01 February 2012 2,801 4 View

NCBI : This record was replaced with Gene ID: 17386

I was looking for the primer of "MMP1" of mouse, yet it shows the message as the title and redirect me to the page named "MMP13", does it means they are the same in mouse?

01 February 2012 7,101 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

Do we put respondents data in research methods or findings section of a qualitative study?

Are both ways acceptable, or is there any convention to them? Thanks.

22 July 2024 6,988 4 View

Restaurant research, methods of quantitative and qualitative statistics?

Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...

20 July 2024 2,033 7 View