What are they??
Are they specific for something??
Does ECM affects cell growth or other properties? Could lacking of ECM or disruption of its structure alter the cell biology?
08 September 2012 7,942 2 View
Has anyone ever extracted protein from tongue tissue to perform ELISA? What kind of lysis buffer did you use?
07 August 2012 6,445 4 View
Does anyone know how to quantify the result of Ki-67 labelling?
06 July 2012 3,366 23 View
Can anyone provide me good primers of TNFa, IL-10, IL-5, IL-13, RANTES, IL-17A for Sybr green qPCR use?
05 June 2012 5,617 4 View
Does anyone know how to deplete mast cell in vivo? Is there any known antibody can serve this purpose?
05 June 2012 2,791 16 View
How can I detect fibrosis within a tissue? Any staining to apply on biopsy or other methods?
05 June 2012 1,781 15 View
To assess the recovery of tongue epithelial cell, what are the marker to look into? Ki67?
04 May 2012 9,093 4 View
In which cells is CD34 expressed? What's its function?
04 May 2012 1,000 13 View
Have anyone used the primer for qPCR to detect cyclooxygenase 2? May I learn the sequence of them??
01 February 2012 2,715 4 View
I was looking for the primer of "MMP1" of mouse, yet it shows the message as the title and redirect me to the page named "MMP13", does it means they are the same in mouse?
01 February 2012 7,028 3 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...
24 February 2021 1,243 1 View
Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...
21 February 2021 5,724 4 View
I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...
12 January 2021 3,606 2 View
Virology, microbiology, immunology, vaccine
21 December 2020 424 16 View
I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...
13 December 2020 5,690 5 View
My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.
06 December 2020 7,964 6 View
A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...
02 November 2020 5,886 6 View
When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...
27 October 2020 2,068 2 View
In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...
26 October 2020 5,966 4 View
