How to store a nitrocellulose membrane after western blot?

01 February 2020 9,382 3 View

How to reduce non-specific interaction without impairing antibody-epitope binding in immunohistochemistry (IHC) ?

Dear expertise, i stained frozen section lung tissue sample against virus protein, which should be detect virus infection. i trying to reduce non-specific interaction using serum (5% BSA in PBS)...

07 August 2019 9,401 2 View

What are the criteria need to apply post-doctoral position ?

Dear Expert, I would like to apply postdoctoral studies. 1. I am wondering how to apply for postdoctoral position especially in the US and Europe school. What are the procedures, I need to go...

06 July 2018 1,022 5 View

What are the OIE- Listed bacterial diseases mostly infecting the shrimp worldwide?

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03 April 2017 6,945 1 View

Could anybody share their protocol for Mycobacterium bacteriophage isolation from aquatic environment?

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03 April 2017 9,679 1 View

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I have done aquatic animal model experiments, which are treated with single, combination of different bacteriophage under laboratory condition, and I controlled bacterial infection by phages. I...

08 September 2016 8,024 11 View

Does anyone know where the facility of Pulsed-field gel electrophoresis for DNA sample in India?

05 June 2016 10,052 1 View

How do I concentrate the PCR products ?

10 November 2015 3,632 25 View

What is the basic method for bacteriophage SDS-PAGE and How do i get all phage structural protein bands ?

1. I am getting streaking with two big bands, two normal bands two/ three very small bands only between 70-40 kDa in 12 % SDS-PAGE gel (CBB strain). but i unable to remove streaking. Is there any...

08 September 2015 4,827 2 View

How to screen out for GAL gene containing bacteriophage from the plagues ?

03 April 2015 556 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View