Is there a simple biochemical assay for lytic polysaccharide monooxygenases (LPMO)?

I need a simple protocol for the assay of LPMO enzymes using biochemical assays just like those of cellulases. Most of those I have seen in the literature used mass spectrometry. I would be...

06 July 2017 8,389 3 View

How should negative OD readings be processed in DNS assay for reducing sugar?

Sometimes I get negative OD readings (at 540 nm) when determining reducing sugars using glucose as standard (Miller, 1959). When I use Microsoft Excel to plot the glucose standard curve, the line...

03 April 2015 4,525 15 View

Why does the specific activity of my enzyme decrease at each step of purification?

Can anyone provide possible reasons why the specific activity of my cellulase (from bacteria) keeps reducing after each step of purification? So far, the crude enzyme has the highest activity...

11 December 2014 8,944 34 View

Why is enzyme not precipitating even at 90% ammonium sulphate saturation?

I am trying to precipitate a bacterial cellulase produced on a lignocellulosic hydrolysate as the first step in the purification of the enzyme. I did step-wise ammonium sulphate fractionation at...

07 August 2014 4,331 18 View

What is the most suitable column to analyze the composition of lignocellulosic hydrolysate using LCMS?

The use of HPLC might give some unknown peaks. However, LCMS would allow a more detailed identification of the compounds in the hydrolysate. Such compounds might include sugars (monomers and...

07 August 2014 5,385 4 View

Is it compulsory to deduct OD readings of enzyme blanks in cellulase assays?

One of the most cited references regarding cellulase (saccharifying cellulase, endo-, exo- and betaglucosidase) assays (Ghose, 1987 & Wood and Bhat, 1988) recommend that OD readings of enzyme...

03 April 2014 6,141 1 View

Conflict between BIOLOG and 16S rRNA sequence BLAST in identifying a bacterial strain?

A bacterium was identified by BIOLOG as a particular species; running a BLAST search on the 16S rRNA sequence of the same organism gave a different specie of the same genus. The one with the...

31 December 2013 1,706 28 View

Can someone advise on the enzyme concentration to release 2 mg glucose in cellulase filter paper assay?

The IUPAC procedure for determining total cellulase activity using filter paper states that one should prepare two dilutions of the enzyme such that one dilution will release > 2 mg glucose from...

10 November 2013 3,109 5 View

How to perform life cycle analysis (LCA)?

I wish to know how to perform life cycle analysis or assessment (LCA) of any biotechnological processes especially for biofuels. What are the basic things to consider? Is there any book or...

08 September 2013 7,831 3 View

Does keeping of broth samples at -20degC affect cfu, OD and enzyme activity readings?

In running an experiment to plot growth curve of an organism which is growing on a cellulosic substrate, it is necessary to collect samples from the shake flask at intervals (2-4 hours over 72...

06 July 2013 7,926 10 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View