Do bacteria (esp. lactobacillus) produce cellulase enzyme naturally?

More Mushafau Adebayo Oke's questions See All

Is there a simple biochemical assay for lytic polysaccharide monooxygenases (LPMO)?

I need a simple protocol for the assay of LPMO enzymes using biochemical assays just like those of cellulases. Most of those I have seen in the literature used mass spectrometry. I would be...

06 July 2017 8,543 3 View

How should negative OD readings be processed in DNS assay for reducing sugar?

Sometimes I get negative OD readings (at 540 nm) when determining reducing sugars using glucose as standard (Miller, 1959). When I use Microsoft Excel to plot the glucose standard curve, the line...

03 April 2015 4,778 15 View

Why does the specific activity of my enzyme decrease at each step of purification?

Can anyone provide possible reasons why the specific activity of my cellulase (from bacteria) keeps reducing after each step of purification? So far, the crude enzyme has the highest activity...

11 December 2014 9,435 34 View

Why is enzyme not precipitating even at 90% ammonium sulphate saturation?

I am trying to precipitate a bacterial cellulase produced on a lignocellulosic hydrolysate as the first step in the purification of the enzyme. I did step-wise ammonium sulphate fractionation at...

07 August 2014 4,490 18 View

What is the most suitable column to analyze the composition of lignocellulosic hydrolysate using LCMS?

The use of HPLC might give some unknown peaks. However, LCMS would allow a more detailed identification of the compounds in the hydrolysate. Such compounds might include sugars (monomers and...

07 August 2014 5,516 4 View

Is it compulsory to deduct OD readings of enzyme blanks in cellulase assays?

One of the most cited references regarding cellulase (saccharifying cellulase, endo-, exo- and betaglucosidase) assays (Ghose, 1987 & Wood and Bhat, 1988) recommend that OD readings of enzyme...

03 April 2014 6,282 1 View

Conflict between BIOLOG and 16S rRNA sequence BLAST in identifying a bacterial strain?

A bacterium was identified by BIOLOG as a particular species; running a BLAST search on the 16S rRNA sequence of the same organism gave a different specie of the same genus. The one with the...

31 December 2013 1,842 28 View

Can someone advise on the enzyme concentration to release 2 mg glucose in cellulase filter paper assay?

The IUPAC procedure for determining total cellulase activity using filter paper states that one should prepare two dilutions of the enzyme such that one dilution will release > 2 mg glucose from...

10 November 2013 3,252 5 View

How to perform life cycle analysis (LCA)?

I wish to know how to perform life cycle analysis or assessment (LCA) of any biotechnological processes especially for biofuels. What are the basic things to consider? Is there any book or...

08 September 2013 8,001 3 View

Does keeping of broth samples at -20degC affect cfu, OD and enzyme activity readings?

In running an experiment to plot growth curve of an organism which is growing on a cellulosic substrate, it is necessary to collect samples from the shake flask at intervals (2-4 hours over 72...

06 July 2013 8,054 10 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

Has anyone used or is familiar with an anti-C4 complement factor antibody for Western Blotting?

Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...

17 June 2024 6,124 1 View

Does age of a bacterial culture have an impact on its DNA isolation?

Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...

13 May 2024 3,061 11 View

Why does soybean half-seed transformation use 1/10x B5 salts during co-cultivation?

I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...

07 May 2024 3,924 0 View

Why I am not getting accumulated GFP in vacuole for om45 WT GFP yeast strains?

I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...

04 May 2024 6,463 0 View

What are the genetic mechanisms governing the production of secondary metabolites in microorganisms?

How are these exploited in biotechnological applications?

07 April 2024 566 5 View

Kishore babu Turlapati

Can any one help, what are the exepients used for solid formulation of feed enzymes in crude form?

Andrea Strazzulli

Theoretically Lactobacillus sp. can hydrolyze cellulose and xylooligosaccharides.

Article Cloning and expression of cellulase and xylanase genes in La...

Mushafau Adebayo Oke

Thank you.