25 Questions 33 Answers 0 Followers
Questions related from Manohar Raju
Typical neutralization buffer used for Protein A/G affinity separations is 1 M Tris/HCl pH 9.0 Any alternate buffer for 1 M Tris/HCl ?
05 March 2024 612 2 View
Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .
05 February 2021 5,978 3 View
Can one explain the reaction between DTSSP - (3,3'-dithiobis(sulfosuccinimidyl propionate)) and Sodium carbonate. It is been mentioned in most of the articles, especially for protein...
16 July 2020 3,842 0 View
Hi all, I am trying to immobilize Antibody by DTSSP on a gold substrate, The DTSSP has a center disulfide. Most of the research articles have not reported the reducing of DTSSP...
27 November 2019 9,012 1 View
Hi all, Am in search of the technical details for Escherichia coli (O111: B4 ) which are as follows 1. Antigen (Epitope) details of O - antigen of O111:B4. 2. Is E coli O111: B4...
17 November 2019 5,963 4 View
Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...
28 May 2019 3,185 1 View
Most of the researchers have reported the use of Redox indicator Methylene blue to distinguish between ssDNA and dsDNA, however in some articles after hybridization the reduction peak was...
21 May 2019 2,007 0 View
Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...
21 December 2018 8,784 2 View
I would like to know if it is possible to separate flagella and cilia from whole cell bacteria.
03 August 2018 7,352 0 View
Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...
15 June 2018 7,364 4 View
Hi, Am working on Biosensors, for that i had used the new gold electrode which comes polished and cleaned. Once am done with an experiment i have to use the same electrode, so i need to...
19 April 2018 4,503 8 View
Hi, How difficulty is to remove the AuNp's coated on to the Gold electrode surface, can it be removed completely 100% ? Can one remove the AuNp's by Polishing with Alumina Powder and...
12 April 2018 9,573 3 View
Am working on DNA Hybridization studies, there are Colorimetric (Photobiotin) studies or DOT blot by which the detection can be done. Any possibilities of using an FTIR analysis for ssDNA and...
04 March 2018 4,098 2 View
Hi, I have been working on Bacillus thuringiensis, when i amplify the Cry1 gene using genomic DNA i usually get a positive result. i need to know whether these Cry gene's are Genomic or...
26 October 2017 4,436 4 View
Hi Everyone, Am looking for a Methods / Protocols for Electrochemical Biosensors, can some one shed the light on these aspects,since am new to Electrochemical biosensors , my aim is to build a...
15 September 2017 2,447 3 View
Any Colony morphology difference in strains of Pseudomonas fluorescence and Pseudomonas fluorescence CHA0?
20 May 2017 2,345 22 View
Hello Everyone, I tried to revive 3years old Pseudomonas fluorescens from glycerol stocks which were at -20O C , these culture failed to grow on Kings B broth and even...
10 April 2017 1,328 5 View
Hi Everyone, i have a Sequence of 16s rRNA, when i tried to generate CAP- Contig using BioEdit, am getting Multiple Contigs for a particular strain, but when i tried with some other strain am...
11 January 2017 697 2 View
Am working on Bacillus thuringiensis, I need to purify the Parasporal Crystals for Bioassay works, any efficient way to isolate protein crystals that should exclude spores ?.
15 October 2016 6,851 6 View
Am working on Bacillus thuringiensis, I wanted to work on Bioassay using Bt against Insects , Is there any Manual or Books for the Procedure of Bioassay , that has Information to calculate on...
19 August 2016 1,263 3 View
Am using an ~ 6 years old Primers now, and am not able to get any amplicons, can this be due to degradation of nucleotides over time ? .
17 June 2016 9,434 9 View
Nanotechnology on Proteins for Larvicial activity and the equipments used for Nanotechnology.
16 May 2016 4,268 3 View
1.What is the Colony Morphology of Bacillus thuringiensis, If possible post a Pic of culture plate. 2. How does Cry Proteins and Spores look like after Staining. 3.Better Method to isolate DNA...
06 April 2016 6,579 3 View
Hi Everyone, I am trying to check for Gene Expression in one of the Bacteria(prokaryote), so i had planned for RNA-Sequencing i.e. Transcriptome Analysis.So, 1.Is it possible to isolate m-RNA...
01 January 1970 8,042 5 View
I have obtained sequencing data for Forward and Reverse Primers, intending to use them in the construction of a Phylogenetic tree. Initially, I have aligned the Forward sequence with the reverse...
01 January 1970 5,311 5 View
