Hi Everyone,
I am trying to check for Gene Expression in one of the Bacteria(prokaryote), so i had planned for RNA-Sequencing i.e. Transcriptome Analysis.So,
1.Is it possible to isolate m-RNA with or without commercial kits, how about the efficiency of the isolated product,any procedure available for isolation of m-RNA from Prokaryotes!
2.How to proceed with the isolated product to construct cDNA, should i follow the conventional RT- PCR, which are the Primers/Enzyme can be used ?
3.Once cDNA has been constructed, can i sequence it for further analysis .