Is it possible to separate flagella from bacteria ?

Manohar Raju @Manohar-Raju-2

03 August 2018 0 7K Report

I would like to know if it is possible to separate flagella and cilia from whole cell bacteria.

Badges
Science topic

More Manohar Raju's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

TEM cell structure: What is this structure in Euglena gracilis?

There are two TEM images of Euglena gracilis, but I am not sure what this structure is? It seems a bit like flagellum/paraflagellar body in the reservoir from my perspective... Is anyone familiar...

15 November 2023 6,074 0 View

How to remove mucus layer from primary nasal epithelial cells in air-liquid interface cultures?

We are currently using the air-liquid interface technique to differentiate human primary nasal epithelial cells. While the cells are differentiating well and producing cilia, some are also...

13 March 2023 5,389 1 View

Is it essential to add 0.1 or 0.2% FBS in DMEM while inducing cilia formation under starvation?

I tried different combinations of starvation situations with RPE-1 cells, but used serum free DMEM while starvation (as mentioned in some of the related papers of the field). But I didn't get...

19 January 2023 2,830 2 View

IBMX+forskolin stimulation of olfactory sensory neurons does not work?

Dear fellow olfactory scientists and/or electrophysiologists, For one of my projects, I would like to stimulate/activate different primary olfactory transduction cascade stations. As a first...

16 January 2023 8,014 0 View

What type of protists are these from my saltwater fish tank?

What type of protists are these from my saltwater fish tank? See file. Also, how can you observe cilia or flagella without oil immersion (1,000x total mag)?

04 January 2023 5,109 1 View

Do B-Cells Have Primary Cilium?

Hello everyone, Does anyone know if B cells have primary cilium? If so, I would be thankful if you can refer me to the paper showing it. And if they do not, then how can they have Hedgehog...

10 October 2022 1,670 0 View

How to prepare lateral flagella for TEM viewing?

My bacterium is Aeromonas dhakensis and I had observed very pretty lateral (peritrichous) flagella under oil immersion light microscope using traditional flagellar stain after growing my bacteria...

14 June 2022 3,518 2 View

Why the motility of V.parahaemollyticus decreases with increased NaCl concentrations?

The polar flagellum is Na+-driven. But the result shows that the swimming ability of V.parahaemollyticus decreases with increased NaCl concentrations

13 May 2022 4,294 0 View

What protocol to use to observe bacteria without the outer-membrane by EM ?

Hi, I would like to look at bacteria devoid of their outer membrane by EM (negative staining). I work on Spirochetes, and this would allow me to see the presence or absence of periplasmic flagella...

16 December 2021 4,477 2 View

How to unfold Protein using a ANTI-FLAG M2 Affinity Gel?

I am currently using the Anti-Flag Affinity Gel to pull down a Flag protein inserted on the c-terminal of a specific protein. I have been unable to detect the flag protein via SDS page. Recently,...

31 October 2021 8,735 3 View