Hi,

I have been working on Bacillus thuringiensis, when i amplify the Cry1 gene using genomic DNA i usually get a positive result. i need to know whether these Cry gene's are Genomic or Plasmid borne ?. I have never tried amplification using Plasmids. Has anyone tried to amplify Cry gene on plasmid isolated from Bacillus thuringiensis.

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