I am working on bacterial-fungal biofilm. I am testing the biofilm formation through crystal violet assay using microtitre plate. My doubt is, i am getting good biofilm formation between bacterium-fungi in microtitre plates, but for the same treatment in broth culture, the yield of EPS is low and there is no correlation between the results of microtitre plate and broth culture with respect to EPS. As per the literature, cellulose-chitin interaction is necessary for biofilm formation in bacterium-fungi biofilm. But, i am not getting good biofilm yield in flask (broth - 150 ml) as compared to microtitre plates (200 micro litres).
Is space is the problem or rapid attachment of bacteria to fungal mycelia retards polysaccharide production?.
Can someone kindly clarify me on this aspect.
What about the bacterium chitinase activity? Aeromonadas, vibrios... they use the chitin semidegradation to colonize.
The comment on the surface is very important: what is the chitin particle size in the batch culture?
We were working with chitin/bacteria/ciliates in continuous-flow system and the colonization was perfect. However, much better in the presence of bacteria-predators. Is your culture protist-free? Flagellates could induce the colonization and if you are not checking the system purity frequently (e.g., with DAPI staining), it may happen (to everybody)
Cheers
Which microtiterplate did you use? high binding? low binding? The surface of culture flask is in any case low binding.
Thanks for your interest.
I am using Polystyrene non treated plates with flat bottom.
Is rapid attachment of bacterium to filamentous fungus hamper bacterial polysaccharide production?. Why because, i am getting good bacterial and fungal population in biofilm formed as compared to planktonic culture, but EPS yield is not comparable to population density
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