How to purify the crude extracellular polysaccharide extracted from microbial biofilm?

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We have done whole transcriptome sequencing of bacteria and fungi. Is there any simple tutorial to submit these NGS data to NCBI.

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How to detect and quantify quorum sensing without using reporter bacteria?

I am interested in quantifying quorum sensing in microbial biofilms. Is there any simple procedure to do so without using reporter bacteria

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How long can we store plant samples in Glutaraldehyde for SEM analysis?

I have to do SEM analysis of root samples colonized by a selected bacterium. After removal of roots from the pots, how long I can store the samples in 2.5% Glutaraldehyde solution. Is there any...

08 December 2016 5,534 6 View

How to store the extracted extracellular and intracellular polysaccharides?

In my biofilm study, i have extracted EPS and IPS. Different protocols are available on their storage (ultra pure water, buffers, distilled water, solvents etc.). Can anyone provide me simple...

14 July 2016 1,203 6 View

How to prepare Rhodamine B and Alcian Blue dyes for biofilm study?

How to prepare Rhodamine B and Alcian Blue dyes for biofilm studies? What should be the solvents, concentration and storage procedure for the dyes?

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How to prepare and store Amino acid stock solution?

Which is the best solution to prepare amino acid stock solution? Sodium hydroxide/KOH/Distilled water?. After preparation, which will be the best condition to store amino acid solution and how...

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Can anybody provide me with a formula to prepare 10mM concentration of amino acid solution from 1mg stock?

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Can anyone help in providing protocol to quantify glyphosate herbicide degradation in vitro through calorimetry or spectrophotometry?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

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What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

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How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

Given the current level of natural phenomena cause by the climate change and environmental pollution, will the AI find the technological solutions?

AI-driven technologies offer previously unheard-of capabilities to process enormous volumes of data, extract insightful knowledge and improve predictive models, according to the UN’s World...

31 July 2024 9,733 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

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What roles do microbial communities play in the environment and roles played by microorganisms in our environment?

25 July 2024 1,992 2 View

Why microbes are used in environmental engineering and role of microbes in reducing environmental pollution and improving environmental quality?

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How do bacterial species contribute to ecosystem functions and nutrient cycling and role of microbial communities for sustainability?

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