Is it possible to quantify rhizodeposits without isotope labeling?

Kulandaivelu Velmourougane @Kulandaivelu-Velmourougane

22 August 2023 0 7K Report

Generally the rhizodeposition and its effects on soil-plant-microbial functions are quantified through isotope labeling. Is there any technique to quantify rhizodeposits without isotope labeling

Badges
Science topic

More Kulandaivelu Velmourougane's questions See All

How to submit whole transcriptome RNAseq to NCBI?

We have done whole transcriptome sequencing of bacteria and fungi. Is there any simple tutorial to submit these NGS data to NCBI.

17 June 2017 1,011 5 View

How to detect and quantify quorum sensing without using reporter bacteria?

I am interested in quantifying quorum sensing in microbial biofilms. Is there any simple procedure to do so without using reporter bacteria

05 May 2017 5,756 4 View

How long can we store plant samples in Glutaraldehyde for SEM analysis?

I have to do SEM analysis of root samples colonized by a selected bacterium. After removal of roots from the pots, how long I can store the samples in 2.5% Glutaraldehyde solution. Is there any...

08 December 2016 5,534 6 View

How to store the extracted extracellular and intracellular polysaccharides?

In my biofilm study, i have extracted EPS and IPS. Different protocols are available on their storage (ultra pure water, buffers, distilled water, solvents etc.). Can anyone provide me simple...

14 July 2016 1,203 6 View

How to prepare Rhodamine B and Alcian Blue dyes for biofilm study?

How to prepare Rhodamine B and Alcian Blue dyes for biofilm studies? What should be the solvents, concentration and storage procedure for the dyes?

15 January 2016 1,135 2 View

How to prepare and store Amino acid stock solution?

Which is the best solution to prepare amino acid stock solution? Sodium hydroxide/KOH/Distilled water?. After preparation, which will be the best condition to store amino acid solution and how...

08 January 2016 8,009 23 View

Can anybody provide me with a formula to prepare 10mM concentration of amino acid solution from 1mg stock?

Preparation of mM concentrations of amino acids

03 December 2015 1,363 7 View

Can anyone help in providing protocol to quantify glyphosate herbicide degradation in vitro through calorimetry or spectrophotometry?

Glyphosate degradation.

13 November 2013 710 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Can I use Polyjet after its expiration date?

I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?

23 July 2024 8,059 1 View

Why is there a significant edge deviation in radar point cloud and camera registration?

The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...

23 July 2024 7,479 3 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

Is the wash in my IP the problem or something else?

The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....

01 July 2024 3,183 0 View

Collecting/storing plant stable isotope samples?

Hello! I am planning to collect plant leaf samples for stable isotope analysis. I will be collecting them from the field and storing fresh tissue on ice. I have heard that bringing along a food...

01 July 2024 9,125 1 View

How to properly label fish feed pellets with stable isotopes for tracing C and N flow?

Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...

19 June 2024 746 0 View

Method to prevent crosstalk fluorescene signal between 2 dye labeled microparticles?

My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....

19 June 2024 2,680 1 View

Please share the HNMR sample preparation procedure for isotopic labelling test to confirm source of N2 during ENRR to ammonia.?

During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.

29 May 2024 5,165 0 View