What is the differences between bottom-up and top-down proteomics approach?

31 December 2016 4,628 3 View

Can creatine kinase M-type protein(43 kDa) be separated in 2DE in around 39 kDa?

I separated proteins from porcine muscle tissue, and I noticed that three spots from CK-m type protein were resolved in around 39 kDa. My question is there any species or modified form of this...

06 July 2016 7,107 3 View

What is the difference between LFQ intensity and plain intensity?

06 July 2016 8,224 4 View

How I can calculate the number of oxidation peptides properly from the proteome discoverer list?

I tried to calculate the total number of oxidation peptides but I noticed that on the list same oxidation peptides were given twice or even the third time for the same peptide.What does this...

04 May 2016 1,394 2 View

I have Graph which was copied from PDF file, How can I add color to the each peak line instead of black color line.Any free software?

I attached the graph picture.I want to color the line of each peak with a different color that I can differentiate between them.The graph copied fro PDF file.Any free software that help?Please...

04 May 2016 8,534 4 View

Waters nanoAcquity UPLC system given a message: system over pressure, what does this mean? and how can i solve the problem?

I performed LC-MS/MS measurements by injecting my samples on nano liquid chromatography system (nanoACQUITYy, Waters, Manchester, UK) coupled via ESI to a quadrupole-orbitrap mass spectrometer...

04 May 2016 4,630 2 View

Is there any effect of iron (Fe) if its present in the protein solution dissolved in PBS buffer?

I performed homogenization of pancreas tissue for protein extraction by using 1X PBS(phosphate buffer saline)at physiological PH,I transferred the tissue from a tube by old forceps,I notice that...

02 March 2016 2,732 3 View

Can anybody help me in letter sample for a journal asking for permission to use figures in the paper?

01 February 2016 2,196 0 View

What are the best sites or free softwares for rephrase sentences?

During writing the thesis, I'm looking for some sites or free software that can help in rephrasing some sentences used in scientific papers.Any recommendation?and is it effective to use such sites...

01 February 2016 7,258 0 View

Can anybody provide me with papers using only PBS with protease inhibitor as buffer lysis for protein extraction from mammalian tissue?

I have used PBS with protease inhibitors as lysis buffer to extract proteins from mamalian tissue,i choosed this buffer that not contain any detergent affect on my results on mass spectrometry.Is...

01 February 2015 5,466 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View