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Questions related from Enrique Espinosa
I am looking for good resources of phisiological plasma concentrations of diverse cytokines in humans. I want to compare them with those of a cohort of HIV+ patients (very late presenters). The...
28 April 2019 9,330 3 View
Appart of measuring intracellular NAD concentration, for which there is a flow cytometry method or use lysates, I have to measure NAD in human plasma samples. What method, kit, brand would you...
07 February 2019 6,034 0 View
When I gate singlets, lymphocytes, CD3 and then CD8 in my cytometry analysis, I see that CD8+ lymphocytes are divided in two. One population is very bright, and the other is less bright although...
28 January 2019 6,921 4 View
I have discordant findings using DAPI and propidium iodide. Should I trust any over the other? I study human memory CD4 T cells.
06 September 2018 9,282 2 View
I need to measure intracellular NAD (total and NAD+) in human T cells. I see there are several systems (colorimetric and ELISA), and I need a kit that is reliable. I don't want to use HPLC for...
09 August 2018 1,724 1 View
1 micro-molar NAD can elicit death in mice T cells. This concentration is 20 times lower than NAD concentration in human plasma. Additionally, NAD is important for T cell survival, which is at...
31 March 2018 10,085 1 View
Do Jurkat and other CD4 T cell lines express CD38? Ideally I would like to have a CD38neg and a CD38pos line. I got lost in the ATCC web page trying to find subclones of the main cell lines. I...
18 September 2017 5,623 2 View
As many other researchers, I'm having a hard time measuring plasma lipopolysaccharide levels by LAL (limulus amoebocyte assay). I will try ELISA. I have found two manufacturers: Aviva Biosystems...
26 June 2017 8,274 1 View
As many other researchers, I'm having a hard time measuring plasma LPS levels by the LAL (limulus amoebocyte assay) method. If ELISA is more consistent and precise it would be a solution. I...
22 March 2017 5,790 1 View
We stimulate purified CD4+ T cells in vitro with agonistic anti-CD3 and anti-CD28 monoclonal antibodies bound to microspheres. I am wondering if the usage of an anti-CD4 antibody during cell...
06 October 2016 5,751 0 View
We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...
13 September 2016 537 6 View
I am using Affymetrix's GeneChip Human Gene 1.0 ST Array, with which I obtained a list of differentially expressed genes in a certain setting. In that list I seesome genes that have different...
26 July 2016 5,342 2 View
LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...
19 May 2016 6,532 5 View
I have access to peripheral blood mononuclear cells that have been cryoprepreserved and stored in liquid nitrogen for 10 years. They belong to an interesting cohort. Cells that have been...
17 December 2015 5,859 12 View
The plasma samples were obtained from 2005 to 2009, and have been stored at -80°C since. I know that many proteins are stable at this tempreature for long periods, but I don't know about small...
25 November 2015 2,357 3 View
I am being offered an expirated kit for the determination of LPS by the method using amoebocyte lysate. Some times some reagents are very stable, even beyond the declared expiration date. This is...
21 October 2015 6,513 3 View
We need to determine NAD (total: NAD + NADH) concentrations in plasma, supernatants from cell cultures, and in cell lysates. We recently had a very bad experience with a kit from another...
22 June 2015 7,377 1 View
I'm running an experiment in whole PBMCs, from which I need to identify CD4 and CD8 T cells, as well as monocytes by flow cytometry. I am familiar with the markers and gating strategies required...
19 May 2015 7,129 8 View
We have plasma samples kept in liquid nitrogen for seven years. Can they still be used to measure cytokines? We are very interested in measuring IL-1, IL-18, and any other related cytokine (IL-6,...
17 April 2015 7,127 4 View
We want to quantify LPS (endotoxin) levels in human plasma. There are several assays from Lonza, of which QCL-1000 (endpoint, chromogenic, based on Limulus amoebocyte lysate) is the most amenable...
09 March 2015 306 3 View
We want to measure any bacterial product in human plasma, since translocation of bacterial material to blood occurs in several conditions, like HIV infection. However, we get very contrasting...
09 February 2015 5,400 4 View
We measured the induction of a series of genes in purified subsets of T cells cells, by polyclonal stimulation through the TCR. Since the conditions varied slightly between experiments, we...
04 November 2014 568 4 View
There are many markers of endothelial activation and damage (sVCAM-1, von Wilebrand factor, D-dimer, etc. etc. etc.), and I need to decide for one (ideally) or two to measure in human blood that...
14 October 2014 786 15 View
Is there an agent for virus inactivation that can be used in whole blood samples, that still allows to make routine tests in those samples, like blood cell count and blood chemistry?
09 October 2014 3,094 1 View
Is there a signaling pathway or a particular signal induced by arrest between S phase and mitosis (cells don't divide after synthesizing new DNA) that initiates cell death? Is this death...
05 September 2014 556 6 View
Any literature on that?
13 June 2014 5,554 4 View
We are analyzing memory CD4 T cell responses (cytokines, co-stimulatory molecules and chemokine receptors) before and after TCR-mediated stimulation. GAPDH gave nice parallel efficiencies with the...
25 September 2013 4,421 12 View
I am going to use Click-iT Edu reagent and protocol to detect cell proliferation by flow cytometry, on cells staining for extracellular and intracellular proteins. The reagents are compatible with...
08 March 2013 1,033 12 View
I need to sort cells (PBMCs mainly) from patients with untreated HIV, active tuberculosis and pandemic H1N1. So I need the cytometer to fit into a Class II Type A2 Biological Safety Cabinet or...
21 October 2012 3,510 3 View
