I am looking for good resources of phisiological plasma concentrations of diverse cytokines in humans. I want to compare them with those of a cohort of HIV+ patients (very late presenters).
The cytokines I'm studying are:
IL12p70, TNFalfa, IL10, IL6, IL1beta, IL8, IL-18
If you are looking at comparisons of human samples, my suggestion would be for you to do a fresh collection. The reason being... ELISAs and actually any antibody based immunoassay works in a vacuum of sorts. Not all ELISAs uses the same antibody pairing. Not all ELISA protocols are the same. And unless you have built in controls for every plate run, the variations between even the same assay (meaning the same antibody pairs) can be substantial. So... looking at published "normal" ranges won't exactly help you since the reported concentrations are not comparable with another sample run using a different antibody pair.
Now... you can try to get the info from the manufacturer of the kit that you want to use. They might have this information when they did their kit development, they have to have someway to control for lot to lot variations.
Dear collect blood samples from apparently healthy individuals from your area of study and establish your reference range because it is relative based on area of study from there you compare with your case samples.
The cytokine concentration depends on a broad number of factors (age, sex, infection, BMI). As Emmanuel Ifeanyi Obeagu suggested I would take a blood sample of "healthy" individuals that match the ones of your HIV+ cohort for comparability.
Hope this helps
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