We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know which one I should buy.
Myc and c-myc are the same thing, the normal cellular (hence the c) version of this protein, which can be oncogenic when mutated.
The myc tag is a 10-residue sequence derived from c-myc, which is a much larger protein.
If you are looking for antibodies to the myc-tag, make sure they recognize this short sequence, which is Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu.
Myc and c-myc are the same thing, the normal cellular (hence the c) version of this protein, which can be oncogenic when mutated.
The myc tag is a 10-residue sequence derived from c-myc, which is a much larger protein.
If you are looking for antibodies to the myc-tag, make sure they recognize this short sequence, which is Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu.
Adam,
thank you very much indeed for the thorough explanation. I really appreciate it.
As Adam has pointed out, such myc tag fusion proteins utilize a standardized epitope derived from the c-Myc protein. 9E10 is an excellent monoclonal for detecting this epitope tag.
Apart from this epitope tag, you may see references to the oncoproteins c-Myc, N-Myc, and L-Myc. Those are encoded by three different MYC genes, and shouldn't concern you in the context of myc fusion proteins.
C-myc is the cytoplasmic version of myc(myelocytomatosis)protein (mutated version of c-myc)
Mark K Chee Do I understand correctly that N-myc epitope tags are never used? (I'm figuring out the notation 'N-myc' in a plasmid sequence, and I suppose it either means that a c-myc tag is on the N-terminus, or that there is an N-myc tag)
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