Durgesh Pitale

11 Questions 34 Answers 0 Followers

Questions related from Durgesh Pitale

Please suggest me IP grade antibody against Leishmania LPG?

I want to isolate Leishmania donovani LPG interacting host proteins. Kindly share your references for selecting antibody for the same.

04 January 2018 9,370 1 View

Can anybody suggest me protocol for immuno-TEM in human blood derived neutrophils?

I want to perform Immuno-TEM staining in human blood-derived neutrophils for my antigen. My protein of interest will suppose to be localized on autophagosomes. Please share protocol for the...

18 September 2017 8,998 3 View

Can someone suggest a protocol for Phospho-JNK IFA staining in neutrophils?

I am using Phospho-SAPK&JNK pThr183+Tyr185 Antibody (E.665.10) of Thermofisher (Pierce antibody). Protocol followed by me is as follows. 1. PMN fixation with 4% PFA/ 30 min/ 4 degree...

02 December 2015 9,664 3 View

How can I express a protein which contains four rare codons viz. Arg, Leu, Ile and Pro in its sequence?

I am expressing Leishmania protein in bacterial system. This sequence has four rare codons of proline, isoleucine, leucine and arginine. I have tried BL-21 DE3, BL-21 Gold, BL-21 RIL, BL-21 RP,...

15 February 2015 5,863 5 View

Is their any IHC method of ROS and NO detection?

I want to detect ROS and NO generation in primary macrophages. DCF-DA and DAF-FM are the methods used by my lab for ROS and NO detection by Fluorimetric analysis. They do this by trypsinizing the...

01 September 2014 7,860 7 View

Does anybody have done mitotracker and cytochrome 'c' IHC staining in leishmania?

I have tried mitotracker staining of leishmania. It is giving very nice staining when parasites are live. When I fixed leishmania with 4% Paraformaldehyde, it looses its mitotracker staining. Also...

27 August 2014 8,599 7 View

What is the protocol for mitotracker staining of protozoan parasites?

I have tried to stain Leishmania mitochondria with a mitotracker but I am not getting clean staining for mitochondria. I have uses the following protocol for staining: 1) 1 micro liter of 100...

18 June 2014 9,173 2 View

How do I free Leishmania amastigotes, which was previously entrapped inside the THP-1 cells?

I have infected macrophage cell line (THP-1) with Leishmania parasite and now I want entrapped parasite to be used for culturing. How do I let the parasite free from macrophages without harming...

17 May 2014 7,755 2 View

Would you please suggest some manufacturers of oxazine dye CI basic blue 141?

I want to stain for neutrophil granules. Is their any other stain or dye used to stain specifically neutrophils granules?

28 February 2014 459 3 View

Is their any cryopreservation protocol for neutrophils?

I have isolated neutrophils in inactivated state as per experimental need. I am searching for neutrophil cryopreservation protocol. I have used FBS+5%DMSO for cryopreservation. But after revival...

23 January 2014 931 2 View

How do I maintain (human) neutrophil live for at least for 2 days?

I have obtained nature protocol for isolation on pure neutrophil population. I want to do work on infections response in neutrophil but shucked at neutrophil culture.

29 December 2013 7,579 25 View