I have isolated neutrophils in inactivated state as per experimental need. I am searching for neutrophil cryopreservation protocol. I have used FBS+5%DMSO for cryopreservation. But after revival of neutrophils (in RPMI 1640) from cryopreservation, I am hardly getting 40% cell survival. Most of them are activated due to stress, cell death and rupture of granules. I have also tried HBSS (without Ca2+ and Mg2+) still I am getting same result. What should I do in order to get inactivated neutrophil after cryopreservation?
in my experience neutrophils are activated very easily. Cryopreservation unavoidably damages the cells and it is not surprising that you recover only 40%.
Hi Paolo,
I had read this paper about neutrophil cryopreservation. Should I try this protocol? Would you guide me what kind of precaution should I take while reviving these cells?
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