Dera Durgesh Pitale ,

Why don't you try to cloning and expression with that G protein sequence or other leishmania bio-marker gene, and then produce your monoclonal antibody by yourself.

Regards,

T.Pengsakul

With respect to the above comment, because the endonuclease is a conserved protein you would want to do an alignment and find a peptide region in the sequence that is specific for Leishmania and not the host, and try peptide immunization. Or, immunize against the whole protein and screen against leismania endonuclease G. If you need to be able to identify the native protein then you should try the latter approach, or cDNA immunization. If you just want it for SDS-PAGE or westerns then peptide would work. If you don't want to do this work on your own, as it is laborious and 5-6 months long, I would highly recommend aldevron.com (a company in Germany that specializes in DND immunization as well as difficult targets).

Thank you Jennifer and Theerakamol Pengsakul. I will try for it.