I have infected macrophage cell line (THP-1) with Leishmania parasite and now I want entrapped parasite to be used for culturing. How do I let the parasite free from macrophages without harming parasite's viability.
It depends if you want the live leishmania on the promastigote or amastigote form. Just plating the cells at 28 °C for a week in leishmania M199 medium (or even RPMI with serum) is enough to free up the promastigote parasites.
You can refer my article in JoVE "A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line". In this article We have lysed the Leishmania infected THP1 cells and quantify the parasite by alamar blue.
You can contact me for any further detail.
here is link for article
http://www.jove.com/video/4054/a-parasite-rescue-transformation-assay-for-antileishmanial-screening
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