How to model a 2D SAW device in comsol?

I'm trying to figure out to generate SAW in a ceramic piezo substrate in 2D. I'm unable to see a propagating SAW in the device. It would be helpful if someone could provide a barebones comsol...

06 November 2020 9,249 3 View

How to upload the name of notable journal The Business & Management Review (Print) when it is not showing in dropdown list?

I am also unable to upload notable journal The Business & Management Review (Print) with ISSN 2047-2854 (Print) ISSN 2047-2862 (ONLINE) Indexing with: ProQuest, Ebsco Host, EconBiz,...

24 June 2020 3,125 3 View

Can anybody suggest me protocol for immuno-TEM in human blood derived neutrophils?

17 September 2017 8,945 3 View

Nitrogen Gas as cooling fluid that is compressible in nature.so what change made in ANSYS Fluent?

i am simulating cooling phenomenon taking Nitrogen Gas as fluid which is compressible in nature.So what change be made in Fluent setting for the compressible fluid as fluid in the Fluent is...

27 April 2016 6,864 1 View

Can we use Comassie blue for loading control after the immunoblotting and imaging of the primary of interest on membrane?

can we use Comassie blue for loading control after the immunoblotting and imaging of the primary of interest on membrane? Is it reversible?

30 March 2016 875 5 View

How do i prepare the positive control from a purified peptide for western blot?

how do i prepare the positive control from a purified peptide for western blot?

13 December 2015 942 4 View

Can someone suggest a protocol for Phospho-JNK IFA staining in neutrophils?

I am using Phospho-SAPK&JNK pThr183+Tyr185 Antibody (E.665.10) of Thermofisher (Pierce antibody). Protocol followed by me is as follows. 1. PMN fixation with 4% PFA/ 30 min/ 4 degree...

01 December 2015 9,613 3 View

Can anyone help me calculate the heat of fusion in a DSC curve with the example please?

Can anyone help me calculate the heat of fusion in a DSC curve with the example please? Can anyone tell me how to calculate heat of crystallization in this figure?

09 October 2015 2,128 8 View

How can I express a protein which contains four rare codons viz. Arg, Leu, Ile and Pro in its sequence?

I am expressing Leishmania protein in bacterial system. This sequence has four rare codons of proline, isoleucine, leucine and arginine. I have tried BL-21 DE3, BL-21 Gold, BL-21 RIL, BL-21 RP,...

14 February 2015 5,821 5 View

Is their any IHC method of ROS and NO detection?

I want to detect ROS and NO generation in primary macrophages. DCF-DA and DAF-FM are the methods used by my lab for ROS and NO detection by Fluorimetric analysis. They do this by trypsinizing the...

31 August 2014 7,815 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View