I want to isolate Leishmania donovani LPG interacting host proteins. Kindly share your references for selecting antibody for the same.
I would recommend Leishmania LPG Monoclonal Antibody (CA7AE)
http://www.thermofisher.com/1/1/38426-500-μl-leishmania-lpg-antibody-monoclonal-ca7ae.html
I am performing multinomial logit (MNL) on discrete choice data. I wanted to understand how I could improve the value of the R square of my model.
30 March 2024 9,304 2 View
I am in the writing part of conceptual framework for the farmer typology. The complications are Farmers are categorized according to the landholdings - marginal, small, medium and large. These...
11 September 2023 6,483 1 View
Zero-point correction= 0.176636 (Hartree/Particle) Thermal correction to Energy= 0.187447 Thermal correction to Enthalpy= 0.188392...
08 August 2023 4,817 1 View
I have three different data groups and wanted to check whether there exists any difference in the data provided in these three sets.
24 December 2022 8,426 2 View
How to select a scale if I am unsure how precisely my respondents can differentiate the points present in the scale?
04 March 2022 3,950 3 View
After reading a lot through research papers and available literature, I am confused about whether we can perform arithmetic mean on a 5-point Likert type scale which is ordinal in nature. There...
02 March 2022 7,202 3 View
I am analyzing the retinal vasculature using ImageJ angiogenesis analyzer plugin. I wanted to know what do the different colors represent? is it the order of branches? Is there a paper which can...
31 March 2021 3,208 2 View
I would like to know if anyone has suggestions for removal vitreous from fixed eye while performing flat mount imaging.
22 March 2021 3,112 3 View
I'm trying to figure out to generate SAW in a ceramic piezo substrate in 2D. I'm unable to see a propagating SAW in the device. It would be helpful if someone could provide a barebones comsol...
06 November 2020 9,461 3 View
give me a tutorial on adding PDK files to cadence virtuoso, and tell me where should we get free PDK files for FINFET, CNFET & FDSOI FET. Thanks.
05 October 2020 7,664 1 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
..
05 August 2024 4,407 0 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View