How to model a 2D SAW device in comsol?

I'm trying to figure out to generate SAW in a ceramic piezo substrate in 2D. I'm unable to see a propagating SAW in the device. It would be helpful if someone could provide a barebones comsol...

06 November 2020 9,249 3 View

How to upload the name of notable journal The Business & Management Review (Print) when it is not showing in dropdown list?

I am also unable to upload notable journal The Business & Management Review (Print) with ISSN 2047-2854 (Print) ISSN 2047-2862 (ONLINE) Indexing with: ProQuest, Ebsco Host, EconBiz,...

24 June 2020 3,125 3 View

Please suggest me IP grade antibody against Leishmania LPG?

I want to isolate Leishmania donovani LPG interacting host proteins. Kindly share your references for selecting antibody for the same.

03 January 2018 9,323 1 View

Can anybody suggest me protocol for immuno-TEM in human blood derived neutrophils?

17 September 2017 8,945 3 View

Nitrogen Gas as cooling fluid that is compressible in nature.so what change made in ANSYS Fluent?

i am simulating cooling phenomenon taking Nitrogen Gas as fluid which is compressible in nature.So what change be made in Fluent setting for the compressible fluid as fluid in the Fluent is...

27 April 2016 6,864 1 View

Can we use Comassie blue for loading control after the immunoblotting and imaging of the primary of interest on membrane?

can we use Comassie blue for loading control after the immunoblotting and imaging of the primary of interest on membrane? Is it reversible?

30 March 2016 875 5 View

How do i prepare the positive control from a purified peptide for western blot?

how do i prepare the positive control from a purified peptide for western blot?

13 December 2015 942 4 View

Can someone suggest a protocol for Phospho-JNK IFA staining in neutrophils?

I am using Phospho-SAPK&JNK pThr183+Tyr185 Antibody (E.665.10) of Thermofisher (Pierce antibody). Protocol followed by me is as follows. 1. PMN fixation with 4% PFA/ 30 min/ 4 degree...

01 December 2015 9,613 3 View

Can anyone help me calculate the heat of fusion in a DSC curve with the example please?

Can anyone help me calculate the heat of fusion in a DSC curve with the example please? Can anyone tell me how to calculate heat of crystallization in this figure?

09 October 2015 2,128 8 View

How can I express a protein which contains four rare codons viz. Arg, Leu, Ile and Pro in its sequence?

I am expressing Leishmania protein in bacterial system. This sequence has four rare codons of proline, isoleucine, leucine and arginine. I have tried BL-21 DE3, BL-21 Gold, BL-21 RIL, BL-21 RP,...

14 February 2015 5,821 5 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View