Recommended Controls

Set up the following controls at the same way as experimental samples.

Non-cell control - contains growth medium without cells to serve as a negative control to determine culture medium background.

Vehicle control - contains the same solvent used for test compounds to serve as a vehicle control.

Negative control - contains cells but no test compounds.

Positive control - contains cells and known proliferation or cytotoxicity inducer.

The full protocol: https://www.creative-bioarray.com/ldh-cytotoxicity-assay.htm

Good luck.