I have a Roche LDH Cytotoxicity detection kit and want to analyse the percentage of cytotoxicity, what is the role of the several background controls mentioned in the Kit protocol?
what are the precautions one should take ?
Recommended Controls
Set up the following controls at the same way as experimental samples.
Non-cell control - contains growth medium without cells to serve as a negative control to determine culture medium background.
Vehicle control - contains the same solvent used for test compounds to serve as a vehicle control.
Negative control - contains cells but no test compounds.
Positive control - contains cells and known proliferation or cytotoxicity inducer.
The full protocol: https://www.creative-bioarray.com/ldh-cytotoxicity-assay.htm
Good luck.
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